Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.

The metabolic and energetic characterization of the growth of Leuconostoc oenos on glucose-citrate or glucose-fructose mixtures enables the potential role of this bacterium in the wine-making process to be ascertained. Moreover, mixotrophic conditions remain a suitable means for improving biomass pr...

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Main Authors: Salou, P, Loubiere, P, Pareilleux, A
Formato: Artigo
Idioma:en
Publicado em: 1994
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC201503/
https://ncbi.nlm.nih.gov/pubmed/8017930
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spelling pubmed-2015032003-12-01 Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose. Salou, P Loubiere, P Pareilleux, A Appl Environ Microbiol Research Article The metabolic and energetic characterization of the growth of Leuconostoc oenos on glucose-citrate or glucose-fructose mixtures enables the potential role of this bacterium in the wine-making process to be ascertained. Moreover, mixotrophic conditions remain a suitable means for improving biomass productivities of malolactic starter cultures. When the malolactic bacterium L. oenos was grown in batch cultures on complex medium at pH 5.0 with glucose-citrate or glucose-fructose mixtures, enhancement of both the specific growth rate and biomass production yields was observed. While growth was possible on fructose as the sole source of energy, citrate alone did not allow subsequent biomass production. The metabolic interactions between the catabolic pathways of the glucose cosubstrates and the heterofermentation of hexoses led to an increased acetate yield as a result of modified NADH oxidation. However, the calculated global coenzyme regeneration showed that the reducing equivalent balance was never equilibrated. The stimulatory effects of these glucose cosubstrates on growth resulted from increased ATP synthesis by substrate-level phosphorylation via acetate kinase. While the energetic efficiency remained close to 10 g of biomass produced per mol of ATP, the increase in the specific growth rate and biomass production yields was directly related to the rate and yield of ATP generation. 1994-05 /pmc/articles/PMC201503/ /pubmed/8017930 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Salou, P
Loubiere, P
Pareilleux, A
Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
description The metabolic and energetic characterization of the growth of Leuconostoc oenos on glucose-citrate or glucose-fructose mixtures enables the potential role of this bacterium in the wine-making process to be ascertained. Moreover, mixotrophic conditions remain a suitable means for improving biomass productivities of malolactic starter cultures. When the malolactic bacterium L. oenos was grown in batch cultures on complex medium at pH 5.0 with glucose-citrate or glucose-fructose mixtures, enhancement of both the specific growth rate and biomass production yields was observed. While growth was possible on fructose as the sole source of energy, citrate alone did not allow subsequent biomass production. The metabolic interactions between the catabolic pathways of the glucose cosubstrates and the heterofermentation of hexoses led to an increased acetate yield as a result of modified NADH oxidation. However, the calculated global coenzyme regeneration showed that the reducing equivalent balance was never equilibrated. The stimulatory effects of these glucose cosubstrates on growth resulted from increased ATP synthesis by substrate-level phosphorylation via acetate kinase. While the energetic efficiency remained close to 10 g of biomass produced per mol of ATP, the increase in the specific growth rate and biomass production yields was directly related to the rate and yield of ATP generation.
author Salou, P
Loubiere, P
Pareilleux, A
author_facet Salou, P
Loubiere, P
Pareilleux, A
author_sort Salou, P
title Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
title_short Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
title_full Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
title_fullStr Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
title_full_unstemmed Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.
title_sort growth and energetics of leuconostoc oenos during cometabolism of glucose with citrate or fructose.
publishDate 1994
url https://ncbi.nlm.nih.gov/pmc/articles/PMC201503/
https://ncbi.nlm.nih.gov/pubmed/8017930
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