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Formation and Regeneration of Protoplasts of the Actinorhizal Nitrogen-Fixing Actinomycete Frankia
Procedures for forming and regenerating protoplasts of four Frankia strains are described. Cells obtained from growth medium containing 0.1% glycine were digested with lysozyme (250 μg/ml) in a medium containing 0.5 M sucrose, 5.0 mM CaCl(2), and 5.0 mM MgCl(2). Protoplasts were formed during 15 to...
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| Hlavní autoři: | , |
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| Médium: | Článek |
| Jazyk: | en |
| Vydáno: |
1987
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC203601/ https://ncbi.nlm.nih.gov/pubmed/16347266 |
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