Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.

Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selectiv...

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Manylion Llyfryddiaeth
Prif Awduron: Schaeffer, W I, Olson, L D, Barile, M F, Sun, F W
Fformat: Erthygl
Iaith:en
Cyhoeddwyd: 1991
Pynciau:
Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC207274/
https://ncbi.nlm.nih.gov/pubmed/1671671
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id pubmed-207274
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spelling pubmed-2072742003-12-01 Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically. Schaeffer, W I Olson, L D Barile, M F Sun, F W J Bacteriol Research Article Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both DNAs. Thus, it is possible to study the molecular biology of this noncultivable mycoplasma in situ rather than after growth in artificial media, with its potential genetic consequences during adjustment to axenic growth. 1991-02 /pmc/articles/PMC207274/ /pubmed/1671671 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Schaeffer, W I
Olson, L D
Barile, M F
Sun, F W
Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
description Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both DNAs. Thus, it is possible to study the molecular biology of this noncultivable mycoplasma in situ rather than after growth in artificial media, with its potential genetic consequences during adjustment to axenic growth.
author Schaeffer, W I
Olson, L D
Barile, M F
Sun, F W
author_facet Schaeffer, W I
Olson, L D
Barile, M F
Sun, F W
author_sort Schaeffer, W I
title Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
title_short Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
title_full Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
title_fullStr Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
title_full_unstemmed Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
title_sort analysis of mycoplasma hyorhinis dna in the presence of host cells without growing the mycoplasma axenically.
publishDate 1991
url https://ncbi.nlm.nih.gov/pmc/articles/PMC207274/
https://ncbi.nlm.nih.gov/pubmed/1671671
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