Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.

Escherichia coli hupA hypB double mutants that lack HU protein have severe cellular defects in cell division, DNA folding, and DNA partitioning. Here we show that the sfiA11 mutation, which alters the SfiA cell division inhibitor, reduces filamentation and production of anucleate cells in AB1157 hup...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Dri, A M, Rouviere-Yaniv, J, Moreau, P L
Formato: Artigo
Idioma:en
Publicado em: 1991
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC207866/
https://ncbi.nlm.nih.gov/pubmed/2019558
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-207866
record_format dspace
spelling pubmed-2078662003-12-01 Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein. Dri, A M Rouviere-Yaniv, J Moreau, P L J Bacteriol Research Article Escherichia coli hupA hypB double mutants that lack HU protein have severe cellular defects in cell division, DNA folding, and DNA partitioning. Here we show that the sfiA11 mutation, which alters the SfiA cell division inhibitor, reduces filamentation and production of anucleate cells in AB1157 hupA hupB strains. However, lexA3(Ind-) and sfiB(ftsZ)114 mutations, which normally counteract the effect of the SfiA inhibitor, could not restore a normal morphology to hupA hupB mutant bacteria. The LexA repressor, which controls the expression of the sfiA gene, was present in hupA hupB mutant bacteria in concentrations half of those of the parent bacteria, but this decrease was independent of the specific cleavage of the LexA repressor by activated RecA protein. One possibility to account for the filamentous morphology of hupA hupB mutant bacteria is that the lack of HU protein alters the expression of specific genes, such as lexA and fts cell division genes. 1991-05 /pmc/articles/PMC207866/ /pubmed/2019558 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Dri, A M
Rouviere-Yaniv, J
Moreau, P L
Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
description Escherichia coli hupA hypB double mutants that lack HU protein have severe cellular defects in cell division, DNA folding, and DNA partitioning. Here we show that the sfiA11 mutation, which alters the SfiA cell division inhibitor, reduces filamentation and production of anucleate cells in AB1157 hupA hupB strains. However, lexA3(Ind-) and sfiB(ftsZ)114 mutations, which normally counteract the effect of the SfiA inhibitor, could not restore a normal morphology to hupA hupB mutant bacteria. The LexA repressor, which controls the expression of the sfiA gene, was present in hupA hupB mutant bacteria in concentrations half of those of the parent bacteria, but this decrease was independent of the specific cleavage of the LexA repressor by activated RecA protein. One possibility to account for the filamentous morphology of hupA hupB mutant bacteria is that the lack of HU protein alters the expression of specific genes, such as lexA and fts cell division genes.
author Dri, A M
Rouviere-Yaniv, J
Moreau, P L
author_facet Dri, A M
Rouviere-Yaniv, J
Moreau, P L
author_sort Dri, A M
title Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
title_short Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
title_full Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
title_fullStr Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
title_full_unstemmed Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
title_sort inhibition of cell division in hupa hupb mutant bacteria lacking hu protein.
publishDate 1991
url https://ncbi.nlm.nih.gov/pmc/articles/PMC207866/
https://ncbi.nlm.nih.gov/pubmed/2019558
_version_ 1759043640465817600

Registos relacionados