Genetic analysis of the rnc operon of Escherichia coli.

RNase III, an Escherichia coli double-stranded endoribonuclease, is known to be involved in maturation of rRNA and regulation of several bacteriophage and Escherichia coli genes. Clones of the region of the E. coli chromosome containing the gene for RNase III (rnc) were obtained by screening genomic...

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Prif Awduron: Takiff, H E, Chen, S M, Court, D L
Fformat: Erthygl
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Cyhoeddwyd: 1989
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Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC209937/
https://ncbi.nlm.nih.gov/pubmed/2540151
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spelling pubmed-2099372003-12-01 Genetic analysis of the rnc operon of Escherichia coli. Takiff, H E Chen, S M Court, D L J Bacteriol Research Article RNase III, an Escherichia coli double-stranded endoribonuclease, is known to be involved in maturation of rRNA and regulation of several bacteriophage and Escherichia coli genes. Clones of the region of the E. coli chromosome containing the gene for RNase III (rnc) were obtained by screening genomic libraries in lambda with DNA known to map near rnc. A phage clone with the rnc region was randomly mutagenized with a delta Tn10 element, and the insertions were recombined onto the chromosome, generating a series of strains with delta Tn10 insertions in the rnc region. Two insertions that had Rnc- phenotypes were located. One of them lay in the rnc gene, and one was in the rnc leader sequence. Polarity studies showed that rnc is in an operon with two other genes, era and recO. The sequence of the recO gene beyond era indicated it could encode a protein of approximately 26 kilodaltons and, like rnc and era, had codon usage consistent with a low level of expression. Experiments using antibiotic cassettes to disrupt the genes rnc, era, and recO showed that era is essential for E. coli growth but that rnc and recO are dispensable. 1989-05 /pmc/articles/PMC209937/ /pubmed/2540151 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Takiff, H E
Chen, S M
Court, D L
Genetic analysis of the rnc operon of Escherichia coli.
description RNase III, an Escherichia coli double-stranded endoribonuclease, is known to be involved in maturation of rRNA and regulation of several bacteriophage and Escherichia coli genes. Clones of the region of the E. coli chromosome containing the gene for RNase III (rnc) were obtained by screening genomic libraries in lambda with DNA known to map near rnc. A phage clone with the rnc region was randomly mutagenized with a delta Tn10 element, and the insertions were recombined onto the chromosome, generating a series of strains with delta Tn10 insertions in the rnc region. Two insertions that had Rnc- phenotypes were located. One of them lay in the rnc gene, and one was in the rnc leader sequence. Polarity studies showed that rnc is in an operon with two other genes, era and recO. The sequence of the recO gene beyond era indicated it could encode a protein of approximately 26 kilodaltons and, like rnc and era, had codon usage consistent with a low level of expression. Experiments using antibiotic cassettes to disrupt the genes rnc, era, and recO showed that era is essential for E. coli growth but that rnc and recO are dispensable.
author Takiff, H E
Chen, S M
Court, D L
author_facet Takiff, H E
Chen, S M
Court, D L
author_sort Takiff, H E
title Genetic analysis of the rnc operon of Escherichia coli.
title_short Genetic analysis of the rnc operon of Escherichia coli.
title_full Genetic analysis of the rnc operon of Escherichia coli.
title_fullStr Genetic analysis of the rnc operon of Escherichia coli.
title_full_unstemmed Genetic analysis of the rnc operon of Escherichia coli.
title_sort genetic analysis of the rnc operon of escherichia coli.
publishDate 1989
url https://ncbi.nlm.nih.gov/pmc/articles/PMC209937/
https://ncbi.nlm.nih.gov/pubmed/2540151
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