Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.

The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates...

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Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Gruber, H, Kern, G, Gauss, P, Gold, L
Μορφή: Άρθρο
Γλώσσα:en
Έκδοση: 1988
Θέματα:
Research Article
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC211689/
https://ncbi.nlm.nih.gov/pubmed/3056918
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spelling pubmed-2116892003-12-01 Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4. Gruber, H Kern, G Gauss, P Gold, L J Bacteriol Research Article The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates of known sequence and secondary structure. Sequence and structure significantly affected nuclease activity. The properties of the enzyme may explain the requirement for the DexA protein during infection of optA mutant hosts. 1988-12 /pmc/articles/PMC211689/ /pubmed/3056918 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Gruber, H
Kern, G
Gauss, P
Gold, L
Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
description The bacteriophage T4 dexA gene product is required during infection of Escherichia coli strains carrying a mutation in the optA gene. We purified the DexA protein from cells which overproduced the protein. The protein was assayed for nuclease activity on synthetic di- and oligonucleotide substrates of known sequence and secondary structure. Sequence and structure significantly affected nuclease activity. The properties of the enzyme may explain the requirement for the DexA protein during infection of optA mutant hosts.
author Gruber, H
Kern, G
Gauss, P
Gold, L
author_facet Gruber, H
Kern, G
Gauss, P
Gold, L
author_sort Gruber, H
title Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
title_short Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
title_full Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
title_fullStr Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
title_full_unstemmed Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.
title_sort effect of dna sequence and structure on nuclease activity of the dexa protein of bacteriophage t4.
publishDate 1988
url https://ncbi.nlm.nih.gov/pmc/articles/PMC211689/
https://ncbi.nlm.nih.gov/pubmed/3056918
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