MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES

BALB/3T3 cells transformed by the Kirsten sarcoma virus (nonvirus producer BALB/3T3 cells) and mutant cell lines derived therefrom by treatment with bromodeoxyuridine (BrdU) were analyzed for expression of virus-specific RNA using single-stranded DNA transcripts of Rauscher leukemia virus (RLV), a v...

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Main Authors: Tsuchida, N., Shih, M., Gilden, R. V., Hatanaka, M.
Formato: Artigo
Idioma:English
Publicado em: The Rockefeller University Press 1974
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2139702/
https://ncbi.nlm.nih.gov/pubmed/4365514
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spelling pubmed-21397022008-04-17 MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES Tsuchida, N. Shih, M. Gilden, R. V. Hatanaka, M. J Exp Med Article BALB/3T3 cells transformed by the Kirsten sarcoma virus (nonvirus producer BALB/3T3 cells) and mutant cell lines derived therefrom by treatment with bromodeoxyuridine (BrdU) were analyzed for expression of virus-specific RNA using single-stranded DNA transcripts of Rauscher leukemia virus (RLV), a virus activated in one of the cell lines (58-2T), and Ki-SV-specific DNA transcript; the latter transcript after removal of all sequences cross-reactive with RLV RNA. The Rauscher virus DNA detected multiple copies of viral RNA in virus-producing cells (∼2.5 x 10(3)/cell) whether infected with RLV or activated to produce virus with BrdU. Nonproducer (NP) cells and normal BALB cells showed small numbers of RNA genomes (70–250/cell) and only partial saturation of the transcript. The intracellular RNA sedimented at 35S (main peak) with a variable minor peak at 20S with the exception of one mutant cell, M-43-2 (main peak at 26–27S). The 58-2T transcript reacted preferentially in NP cells and their derivatives with biphasic kinetics suggesting the possibility of sequences specific for the original transforming virus. The size of Ki-SV specific sequences were 30S in mutant cells whether or not complete virus was being produced and independent of in vivo transplantability. The Rockefeller University Press 1974-07-01 /pmc/articles/PMC2139702/ /pubmed/4365514 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Article
spellingShingle Article
Tsuchida, N.
Shih, M.
Gilden, R. V.
Hatanaka, M.
MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
description BALB/3T3 cells transformed by the Kirsten sarcoma virus (nonvirus producer BALB/3T3 cells) and mutant cell lines derived therefrom by treatment with bromodeoxyuridine (BrdU) were analyzed for expression of virus-specific RNA using single-stranded DNA transcripts of Rauscher leukemia virus (RLV), a virus activated in one of the cell lines (58-2T), and Ki-SV-specific DNA transcript; the latter transcript after removal of all sequences cross-reactive with RLV RNA. The Rauscher virus DNA detected multiple copies of viral RNA in virus-producing cells (∼2.5 x 10(3)/cell) whether infected with RLV or activated to produce virus with BrdU. Nonproducer (NP) cells and normal BALB cells showed small numbers of RNA genomes (70–250/cell) and only partial saturation of the transcript. The intracellular RNA sedimented at 35S (main peak) with a variable minor peak at 20S with the exception of one mutant cell, M-43-2 (main peak at 26–27S). The 58-2T transcript reacted preferentially in NP cells and their derivatives with biphasic kinetics suggesting the possibility of sequences specific for the original transforming virus. The size of Ki-SV specific sequences were 30S in mutant cells whether or not complete virus was being produced and independent of in vivo transplantability.
author Tsuchida, N.
Shih, M.
Gilden, R. V.
Hatanaka, M.
author_facet Tsuchida, N.
Shih, M.
Gilden, R. V.
Hatanaka, M.
author_sort Tsuchida, N.
title MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
title_short MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
title_full MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
title_fullStr MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
title_full_unstemmed MUTANTS OF NONPRODUCER CELL LINES TRANSFORMED BY MURINE SARCOMA VIRUS : III. DETECTION AND CHARACTERIZATION OF RNA SPECIFIC FOR HELPER AND SARCOMA VIRUSES
title_sort mutants of nonproducer cell lines transformed by murine sarcoma virus : iii. detection and characterization of rna specific for helper and sarcoma viruses
publisher The Rockefeller University Press
publisher_facet The Rockefeller University Press
publishDate 1974
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2139702/
https://ncbi.nlm.nih.gov/pubmed/4365514
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