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Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein.

The introduction of disulfide crosslinks is a generally useful method by which to identify regions of a protein that are close together in space. Here we describe the use of disulfide crosslinks to investigate the structure and flexibility of a family of designed 4-helix bundle proteins. The results...

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Autors principals: Regan, L., Rockwell, A., Wasserman, Z., DeGrado, W.
Format: Article
Idioma:English
Publicat: Cold Spring Harbor Laboratory Press 1994
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142780/
https://ncbi.nlm.nih.gov/pubmed/7756995
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