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Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate.

NMR spectroscopy was used to search for mechanistically significant differences in the local mobility of the main-chain amides of Bacillus circulans xylanase (BCX) in its native and catalytically competent covalent glycosyl-enzyme intermediate states. 15N T1, T2, and 15N[1H] NOE values were measured...

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Bibliografski detalji
Glavni autori: Connelly, G. P., Withers, S. G., McIntosh, L. P.
Format: Članak
Jezik:English
Izdano: Cold Spring Harbor Laboratory Press 2000
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144565/
https://ncbi.nlm.nih.gov/pubmed/10752613
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