Purification, crystallization and preliminary X-ray analysis of the catalytic domain of the Escherichia coli tRNase colicin D

The tRNase domain of colicin D, which cleaves only tRNA(Arg)s at the 3′ side of their anticodon loops, has been expressed in Escherichia coli with its inhibitor protein and purified to a form free from the inhibitor using a low-pH buffer. Crystals were obtained by the hanging-drop vapour-diffusion m...

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Автори:	Takahashi, Kazutoshi, Ogawa, Tetsuhiro, Hidaka, Makoto, Ohsawa, Kanju, Masaki, Haruhiko, Yajima, Shunsuke
Формат:	Стаття
Мова:	English
Опубліковано:	International Union of Crystallography 2005
Предмети:	Crystallization Communications
Онлайн доступ:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2150931/ https://ncbi.nlm.nih.gov/pubmed/16511255 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105039679
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