Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.

The appearance of newly induced LamB protein at the cell surface of Escherichia coli was followed topographically by immuno-electron microscopy. LamB protein was induced in E. coli wild-type or lac-lamB cells for a short period of time (4 to 6 min), such that the overall level of LamB protein in ind...

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Autors principals: Vos-Scheperkeuter, G H, Pas, E, Brakenhoff, G J, Nanninga, N, Witholt, B
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Idioma:en
Publicat: 1984
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC215664/
https://ncbi.nlm.nih.gov/pubmed/6086567
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spelling pubmed-2156642003-12-01 Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells. Vos-Scheperkeuter, G H Pas, E Brakenhoff, G J Nanninga, N Witholt, B J Bacteriol Research Article The appearance of newly induced LamB protein at the cell surface of Escherichia coli was followed topographically by immuno-electron microscopy. LamB protein was induced in E. coli wild-type or lac-lamB cells for a short period of time (4 to 6 min), such that the overall level of LamB protein in induced cells was at least twofold higher than that in uninduced cells. Antibodies bound to LamB protein exposed at the cell surface were labeled with a protein A-gold probe, and the probe distribution in briefly induced cells was compared to that in uninduced cells. Analysis of large numbers of cells showed that newly inserted LamB protein appeared homogeneously over the entire cell surface, both in wild-type cells and in lac-lamB cells. A peak of insertion which was observed at the division site of the cell was also observed in the absence of induction and in control experiments in which a nonspecific probe was used. It is concluded therefore that insertion of LamB protein into the cell envelope of E. coli occurs at multiple sites over the entire cell surface. The average amount of LamB protein which appeared at the cell surface after induction was determined for various cell size classes. It was found that cells of various size classes all synthesized LamB protein after induction, indicating that synthesis of the protein was not restricted to cells in a particular stage of the cell cycle. However, the rate of LamB synthesis was found to vary during the cell cycle: this rate was constant regardless of cell size in nondividing cells, whereas it increased in dividing cells. It is concluded that the accumulation of newly induced LamB protein follows a linear pattern. 1984-08 /pmc/articles/PMC215664/ /pubmed/6086567 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Vos-Scheperkeuter, G H
Pas, E
Brakenhoff, G J
Nanninga, N
Witholt, B
Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
description The appearance of newly induced LamB protein at the cell surface of Escherichia coli was followed topographically by immuno-electron microscopy. LamB protein was induced in E. coli wild-type or lac-lamB cells for a short period of time (4 to 6 min), such that the overall level of LamB protein in induced cells was at least twofold higher than that in uninduced cells. Antibodies bound to LamB protein exposed at the cell surface were labeled with a protein A-gold probe, and the probe distribution in briefly induced cells was compared to that in uninduced cells. Analysis of large numbers of cells showed that newly inserted LamB protein appeared homogeneously over the entire cell surface, both in wild-type cells and in lac-lamB cells. A peak of insertion which was observed at the division site of the cell was also observed in the absence of induction and in control experiments in which a nonspecific probe was used. It is concluded therefore that insertion of LamB protein into the cell envelope of E. coli occurs at multiple sites over the entire cell surface. The average amount of LamB protein which appeared at the cell surface after induction was determined for various cell size classes. It was found that cells of various size classes all synthesized LamB protein after induction, indicating that synthesis of the protein was not restricted to cells in a particular stage of the cell cycle. However, the rate of LamB synthesis was found to vary during the cell cycle: this rate was constant regardless of cell size in nondividing cells, whereas it increased in dividing cells. It is concluded that the accumulation of newly induced LamB protein follows a linear pattern.
author Vos-Scheperkeuter, G H
Pas, E
Brakenhoff, G J
Nanninga, N
Witholt, B
author_facet Vos-Scheperkeuter, G H
Pas, E
Brakenhoff, G J
Nanninga, N
Witholt, B
author_sort Vos-Scheperkeuter, G H
title Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
title_short Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
title_full Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
title_fullStr Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
title_full_unstemmed Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.
title_sort topography of the insertion of lamb protein into the outer membrane of escherichia coli wild-type and lac-lamb cells.
publishDate 1984
url https://ncbi.nlm.nih.gov/pmc/articles/PMC215664/
https://ncbi.nlm.nih.gov/pubmed/6086567
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