European Multicenter Evaluation of Commercial Enzyme Immunoassays for Detecting Norovirus Antigen in Fecal Samples

A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Ge...

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Autors principals: Gray, Jim J., Kohli, Evelyne, Ruggeri, Franco M., Vennema, Harry, Sánchez-Fauquier, Alicia, Schreier, Eckart, Gallimore, Chris I., Iturriza-Gomara, Miren, Giraudon, Helene, Pothier, Pierre, Di Bartolo, Ilaria, Inglese, Nadia, de Bruin, Erwin, van der Veer, Bas, Moreno, Silvia, Montero, Vanessa, de Llano, Marí C., Höhne, Marina, Diedrich, Sabine M.
Format: Article
Idioma:English
Publicat: American Society for Microbiology (ASM) 2007
Matèries:
Clinical and Diagnostic Laboratory Immunology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2168115/
https://ncbi.nlm.nih.gov/pubmed/17715333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00214-07
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Sumari:A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDASCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 96.37%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.

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