Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.

A system for transport of coenzyme M, 2-mercaptoethanesulfonic acid (HS--CoM), in Methanobacterium ruminatium strain M1 required energy, showed saturation kinetics, and concentrated the coenzyme against a gradient. The process was sensitive to temperature and was maximally active at pH 7.1. Cells to...

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Main Authors: Balch, W E, Wolfe, R S
Format: Artigo
Sprog:en
Udgivet: 1979
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Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC218445/
https://ncbi.nlm.nih.gov/pubmed/33148
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spelling pubmed-2184452003-12-01 Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium. Balch, W E Wolfe, R S J Bacteriol Research Article A system for transport of coenzyme M, 2-mercaptoethanesulfonic acid (HS--CoM), in Methanobacterium ruminatium strain M1 required energy, showed saturation kinetics, and concentrated the coenzyme against a gradient. The process was sensitive to temperature and was maximally active at pH 7.1. Cells took up HS--CoM at a linear rate, with a Vmax of 312 pmol/min per mg (dry weight) and an apparent Km of 73 nM. An intracellular pool of up to 5 mM accumulated which was not exchangeable with the medium. Uptake required both hydrogen and carbon dioxide; it was inhibited by O2. Bromoethanesulfonic acid (BrCH2CH2SO3-), a potent inhibitor of methanogenesis in cell-free extracts, inhibited both uptake and methane production. Results of inhibitor studies with derivatives and analogs of the coenzyme showed that the specificity of the carrier is restricted to a limited range of thioether, thioester, and thiocarbonate derivatives. 2-(Methylthio)ethanesulfonic acid (CH3--S--CoM) showed an apparent Ki for HS--CoM uptake of 15 nM, being taken up itself with a Vmax of 320 pmol/min per mg (dry weight) and an apparent Km of 50 nM. An analysis of intracellular pools after HS--CoM uptake indicated that the predominant forms are a heterodisulfide of unknown composition and CH3--S--CoM. 1979-01 /pmc/articles/PMC218445/ /pubmed/33148 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Balch, W E
Wolfe, R S
Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
description A system for transport of coenzyme M, 2-mercaptoethanesulfonic acid (HS--CoM), in Methanobacterium ruminatium strain M1 required energy, showed saturation kinetics, and concentrated the coenzyme against a gradient. The process was sensitive to temperature and was maximally active at pH 7.1. Cells took up HS--CoM at a linear rate, with a Vmax of 312 pmol/min per mg (dry weight) and an apparent Km of 73 nM. An intracellular pool of up to 5 mM accumulated which was not exchangeable with the medium. Uptake required both hydrogen and carbon dioxide; it was inhibited by O2. Bromoethanesulfonic acid (BrCH2CH2SO3-), a potent inhibitor of methanogenesis in cell-free extracts, inhibited both uptake and methane production. Results of inhibitor studies with derivatives and analogs of the coenzyme showed that the specificity of the carrier is restricted to a limited range of thioether, thioester, and thiocarbonate derivatives. 2-(Methylthio)ethanesulfonic acid (CH3--S--CoM) showed an apparent Ki for HS--CoM uptake of 15 nM, being taken up itself with a Vmax of 320 pmol/min per mg (dry weight) and an apparent Km of 50 nM. An analysis of intracellular pools after HS--CoM uptake indicated that the predominant forms are a heterodisulfide of unknown composition and CH3--S--CoM.
author Balch, W E
Wolfe, R S
author_facet Balch, W E
Wolfe, R S
author_sort Balch, W E
title Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
title_short Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
title_full Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
title_fullStr Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
title_full_unstemmed Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium.
title_sort transport of coenzyme m (2-mercaptoethanesulfonic acid) in methanobacterium ruminantium.
publishDate 1979
url https://ncbi.nlm.nih.gov/pmc/articles/PMC218445/
https://ncbi.nlm.nih.gov/pubmed/33148
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