Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin

In the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca(2+)](i)). Ca(2+) binding proteins are enriched in the cerebellum, but their role in inf...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Schiffmann, Serge N., Cheron, Guy, Lohof, Ann, d’Alcantara, Pablo, Meyer, Michael, Parmentier, Marc, Schurmans, Stéphane
Format: Article
Idioma:en
Publicat: The National Academy of Sciences 1999
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC21851/
https://ncbi.nlm.nih.gov/pubmed/10220453
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
id pubmed-21851
record_format dspace
spelling pubmed-218512001-03-09 Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin Schiffmann, Serge N. Cheron, Guy Lohof, Ann d’Alcantara, Pablo Meyer, Michael Parmentier, Marc Schurmans, Stéphane Proc Natl Acad Sci U S A Biological Sciences In the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca(2+)](i)). Ca(2+) binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr(−/−)) are impaired in tests of motor coordination. An impairment in Ca(2+) homeostasis in Cr(−/−) Purkinje cells was supported by the high Ca(2+)-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr(−/−) alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology. The National Academy of Sciences 1999-04-27 /pmc/articles/PMC21851/ /pubmed/10220453 Text en Copyright © 1999, The National Academy of Sciences
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Biological Sciences
spellingShingle Biological Sciences
Schiffmann, Serge N.
Cheron, Guy
Lohof, Ann
d’Alcantara, Pablo
Meyer, Michael
Parmentier, Marc
Schurmans, Stéphane
Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
description In the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca(2+)](i)). Ca(2+) binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr(−/−)) are impaired in tests of motor coordination. An impairment in Ca(2+) homeostasis in Cr(−/−) Purkinje cells was supported by the high Ca(2+)-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr(−/−) alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology.
author Schiffmann, Serge N.
Cheron, Guy
Lohof, Ann
d’Alcantara, Pablo
Meyer, Michael
Parmentier, Marc
Schurmans, Stéphane
author_facet Schiffmann, Serge N.
Cheron, Guy
Lohof, Ann
d’Alcantara, Pablo
Meyer, Michael
Parmentier, Marc
Schurmans, Stéphane
author_sort Schiffmann, Serge N.
title Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
title_short Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
title_full Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
title_fullStr Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
title_full_unstemmed Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
title_sort impaired motor coordination and purkinje cell excitability in mice lacking calretinin
publisher The National Academy of Sciences
publisher_facet The National Academy of Sciences
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC21851/
https://ncbi.nlm.nih.gov/pubmed/10220453
_version_ 1758626110068752384

Ítems similars