Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice

Human systemic lupus erythematosus (SLE) patients, as well as MRLlpr/lpr mice which develop a SLE-like disease, have decreased numbers and functional activity of systemic natural killer (NK) cells. In contrast, it has been found that among lymphocytes recovered from the bronchoalveolar lavage fluid...

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Main Authors: NILSSON, N, CARLSTEN, H
Formato: Artigo
Idioma:English
Publicado em: Blackwell Science Inc 1996
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Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2200532/
https://ncbi.nlm.nih.gov/pubmed/8809138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1046/j.1365-2249.1996.d01-787.x
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spelling pubmed-22005322008-01-16 Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice NILSSON, N CARLSTEN, H Clin Exp Immunol Original Articles Human systemic lupus erythematosus (SLE) patients, as well as MRLlpr/lpr mice which develop a SLE-like disease, have decreased numbers and functional activity of systemic natural killer (NK) cells. In contrast, it has been found that among lymphocytes recovered from the bronchoalveolar lavage fluid of SLE patients, NK cells were increased in number, correlating with the severity of the lung engagement. The present study was undertaken to assay the capacity for natural killing in the lung compartment of MRLlpr/lpr mice compared with healthy congenic MRL +/+ and heterozygous MRL +/lpr mice. (51)Cr-labelled YAC-1 cells were injected intravenously to settle in the lungs where they were targeted for lysis by NK cells. YAC-1 cell killing inversely correlated with radioactivity remaining in the lungs after the assay, and was inhibited by antibody to the asialo-GM1 antigen expressed on NK cells. To analyse the capacity in the lung for cytolysis of non-NK cell-sensitive target cells, a similar in vivo(51)Cr-release assay was set up for antibody-mediated allospecific cytotoxicity. We demonstrate that MRLlpr/lpr mice throughout their lifespan display significantly increased natural cytotoxic activity in the lungs compared with MRL +/+ and MRL +/lpr mice, as demonstrated by more efficient killing of YAC-1 cells. In contrast, antibody-mediated allospecific cytotoxicity in the lungs was significantly less effective in the MRLlpr/lpr strain. Blackwell Science Inc 1996-09 /pmc/articles/PMC2200532/ /pubmed/8809138 http://dx.doi.org/10.1046/j.1365-2249.1996.d01-787.x Text en © 1996 Blackwell Science
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Original Articles
spellingShingle Original Articles
NILSSON, N
CARLSTEN, H
Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
description Human systemic lupus erythematosus (SLE) patients, as well as MRLlpr/lpr mice which develop a SLE-like disease, have decreased numbers and functional activity of systemic natural killer (NK) cells. In contrast, it has been found that among lymphocytes recovered from the bronchoalveolar lavage fluid of SLE patients, NK cells were increased in number, correlating with the severity of the lung engagement. The present study was undertaken to assay the capacity for natural killing in the lung compartment of MRLlpr/lpr mice compared with healthy congenic MRL +/+ and heterozygous MRL +/lpr mice. (51)Cr-labelled YAC-1 cells were injected intravenously to settle in the lungs where they were targeted for lysis by NK cells. YAC-1 cell killing inversely correlated with radioactivity remaining in the lungs after the assay, and was inhibited by antibody to the asialo-GM1 antigen expressed on NK cells. To analyse the capacity in the lung for cytolysis of non-NK cell-sensitive target cells, a similar in vivo(51)Cr-release assay was set up for antibody-mediated allospecific cytotoxicity. We demonstrate that MRLlpr/lpr mice throughout their lifespan display significantly increased natural cytotoxic activity in the lungs compared with MRL +/+ and MRL +/lpr mice, as demonstrated by more efficient killing of YAC-1 cells. In contrast, antibody-mediated allospecific cytotoxicity in the lungs was significantly less effective in the MRLlpr/lpr strain.
author NILSSON, N
CARLSTEN, H
author_facet NILSSON, N
CARLSTEN, H
author_sort NILSSON, N
title Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
title_short Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
title_full Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
title_fullStr Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
title_full_unstemmed Enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of MRLlpr/lpr mice
title_sort enhanced natural but diminished antibody-mediated cytotoxicity in the lungs of mrllpr/lpr mice
publisher Blackwell Science Inc
publisher_facet Blackwell Science Inc
publishDate 1996
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2200532/
https://ncbi.nlm.nih.gov/pubmed/8809138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1046/j.1365-2249.1996.d01-787.x
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