The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra

A new type of biological particle, isolated from the marine dinoflagellate Gonyaulax polyedra, has been partially purified and characterized. When the pH is lowered, the particle emits light in vitro in a fashion closely mimicking the flash of the living cell, and it is referred to as a scintillon (...

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Hoofdauteurs: DeSa, Richard, Hastings, J. Woodland
Formaat: Artikel
Taal:English
Gepubliceerd in: The Rockefeller University Press 1968
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2201157/
https://ncbi.nlm.nih.gov/pubmed/5642469
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spelling pubmed-22011572008-04-23 The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra DeSa, Richard Hastings, J. Woodland J Gen Physiol Article A new type of biological particle, isolated from the marine dinoflagellate Gonyaulax polyedra, has been partially purified and characterized. When the pH is lowered, the particle emits light in vitro in a fashion closely mimicking the flash of the living cell, and it is referred to as a scintillon (flashing unit). Scintillons are obtained by breaking the cells in buffer at pH 8.2 and purifying by differential and sucrose density gradient centrifugation. The particle has a density of about 1.23 g cc(-1), and activity is quantitatively correlated with the number of crystal-like birhombohedral structures. These have been found to contain guanine, but since the density of authentic guanine is about 1.73 g cc(-1), the scintillon is believed to comprise additional but as yet unidentified components. The properties of the scintillon and the effects of various physical and chemical treatments are described. The reasons for believing that this particle is responsible for the flash of the intact cell are discussed. The Rockefeller University Press 1968-01-01 /pmc/articles/PMC2201157/ /pubmed/5642469 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Article
spellingShingle Article
DeSa, Richard
Hastings, J. Woodland
The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
description A new type of biological particle, isolated from the marine dinoflagellate Gonyaulax polyedra, has been partially purified and characterized. When the pH is lowered, the particle emits light in vitro in a fashion closely mimicking the flash of the living cell, and it is referred to as a scintillon (flashing unit). Scintillons are obtained by breaking the cells in buffer at pH 8.2 and purifying by differential and sucrose density gradient centrifugation. The particle has a density of about 1.23 g cc(-1), and activity is quantitatively correlated with the number of crystal-like birhombohedral structures. These have been found to contain guanine, but since the density of authentic guanine is about 1.73 g cc(-1), the scintillon is believed to comprise additional but as yet unidentified components. The properties of the scintillon and the effects of various physical and chemical treatments are described. The reasons for believing that this particle is responsible for the flash of the intact cell are discussed.
author DeSa, Richard
Hastings, J. Woodland
author_facet DeSa, Richard
Hastings, J. Woodland
author_sort DeSa, Richard
title The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
title_short The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
title_full The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
title_fullStr The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
title_full_unstemmed The Characterization of Scintillons : Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra
title_sort characterization of scintillons : bioluminescent particles from the marine dinoflagellate, gonyaulax polyedra
publisher The Rockefeller University Press
publisher_facet The Rockefeller University Press
publishDate 1968
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2201157/
https://ncbi.nlm.nih.gov/pubmed/5642469
_version_ 1760522976911949824

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