Detection of Escherichia coli O157:H7 by multiplex PCR.

In order to develop a PCR assay for Escherichia coli O157:H7, a portion of the 60-MDa plasmid harbored by enterohemorrhagic E. coli (EHEC) was sequenced and PCR primers were designed. A multiplex PCR method was then designed by employing primers specific for the EHEC eaeA gene, conserved sequences o...

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Main Authors: Fratamico, P M, Sackitey, S K, Wiedmann, M, Deng, M Y
Format: Artigo
Jezik:en
Izdano: 1995
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC228363/
https://ncbi.nlm.nih.gov/pubmed/7559976
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https://ncbi.nlm.nih.gov/pmc/articles/PMC228363/
https://ncbi.nlm.nih.gov/pubmed/7559976

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