Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking

In order to evaluate the role of cysteine peptidase cathepsin H (Cath H) in human lung cancer its protein levels were determined in 148 pairs of lung tumour tissue and adjacent non-tumourous lung parenchyma using the enzyme-linked immunosorbent assay technique. Additionally, Cath H levels were deter...

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Main Authors: Schweiger, A, Staib, A, Werle, B, Kras̆ovec, M, Lah, T T, Ebert, W, Turk, V, Kos, J
Formato: Artigo
Idioma:English
Publicado em: Nature Publishing Group 2000
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Regular Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374398/
https://ncbi.nlm.nih.gov/pubmed/10732746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1054/bjoc.1999.0999
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spelling pubmed-23743982009-09-10 Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking Schweiger, A Staib, A Werle, B Kras̆ovec, M Lah, T T Ebert, W Turk, V Kos, J Br J Cancer Regular Article In order to evaluate the role of cysteine peptidase cathepsin H (Cath H) in human lung cancer its protein levels were determined in 148 pairs of lung tumour tissue and adjacent non-tumourous lung parenchyma using the enzyme-linked immunosorbent assay technique. Additionally, Cath H levels were determined in sera of 171 patients with malignant tumours, 34 patients with benign lung diseases and 47 healthy controls. The median level of Cath H in tumour tissue was 0.64 times that in the corresponding lung parenchyma. Relating tumour levels with histological type we found higher Cath H levels in small-cell and adenocarcinomas and lower levels in squamous cell carcinoma, large-cell carcinoma and secondary tumours. A significant difference in Cath H level between lung tumour tissue and non-tumourous lung parenchyma was associated with the group of cigarette smokers (156 vs 263 ng mg(−1)protein, P< 0.001). For this group of patients Cath H tumour levels correlated with the survival rate, while for the entire patient population this was not the case. Smokers with high tumour levels of Cath H experienced poor survival. Cath H was significantly higher in sera of patients with malignant and benign lung diseases than in control sera (P< 0.001). The increase was significant for all histological types, being the highest in small-cell and squamous cell carcinomas. Our study reveals that in lung tumours there is different behaviour of Cath H compared with other cysteine peptidases, e.g. cathepsin B and cathepsin L. Variations between tissue and serum levels of Cath H indicate either reduced expression or enhanced secretion of this enzyme in lung tumours. © 2000 Cancer Research Campaign Nature Publishing Group 2000-02 /pmc/articles/PMC2374398/ /pubmed/10732746 http://dx.doi.org/10.1054/bjoc.1999.0999 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Regular Article
spellingShingle Regular Article
Schweiger, A
Staib, A
Werle, B
Kras̆ovec, M
Lah, T T
Ebert, W
Turk, V
Kos, J
Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
description In order to evaluate the role of cysteine peptidase cathepsin H (Cath H) in human lung cancer its protein levels were determined in 148 pairs of lung tumour tissue and adjacent non-tumourous lung parenchyma using the enzyme-linked immunosorbent assay technique. Additionally, Cath H levels were determined in sera of 171 patients with malignant tumours, 34 patients with benign lung diseases and 47 healthy controls. The median level of Cath H in tumour tissue was 0.64 times that in the corresponding lung parenchyma. Relating tumour levels with histological type we found higher Cath H levels in small-cell and adenocarcinomas and lower levels in squamous cell carcinoma, large-cell carcinoma and secondary tumours. A significant difference in Cath H level between lung tumour tissue and non-tumourous lung parenchyma was associated with the group of cigarette smokers (156 vs 263 ng mg(−1)protein, P< 0.001). For this group of patients Cath H tumour levels correlated with the survival rate, while for the entire patient population this was not the case. Smokers with high tumour levels of Cath H experienced poor survival. Cath H was significantly higher in sera of patients with malignant and benign lung diseases than in control sera (P< 0.001). The increase was significant for all histological types, being the highest in small-cell and squamous cell carcinomas. Our study reveals that in lung tumours there is different behaviour of Cath H compared with other cysteine peptidases, e.g. cathepsin B and cathepsin L. Variations between tissue and serum levels of Cath H indicate either reduced expression or enhanced secretion of this enzyme in lung tumours. © 2000 Cancer Research Campaign
author Schweiger, A
Staib, A
Werle, B
Kras̆ovec, M
Lah, T T
Ebert, W
Turk, V
Kos, J
author_facet Schweiger, A
Staib, A
Werle, B
Kras̆ovec, M
Lah, T T
Ebert, W
Turk, V
Kos, J
author_sort Schweiger, A
title Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
title_short Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
title_full Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
title_fullStr Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
title_full_unstemmed Cysteine proteinase cathepsin H in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
title_sort cysteine proteinase cathepsin h in tumours and sera of lung cancer patients: relation to prognosis and cigarette smoking
publisher Nature Publishing Group
publisher_facet Nature Publishing Group
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2374398/
https://ncbi.nlm.nih.gov/pubmed/10732746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1054/bjoc.1999.0999
_version_ 1760582241620066304

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