Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes

Neurotoxin cluster gene sequences and arrangements were elucidated for strains of Clostridium botulinum encoding botulinum neurotoxin (BoNT) subtypes A3, A4, and a unique A1-producing strain (HA(−) Orfx(+) A1). These sequences were compared to the known neurotoxin cluster sequences of C. botulinum s...

Fuld beskrivelse

Na minha lista:

Bibliografiske detaljer
Main Authors:	Jacobson, Mark J., Lin, Guangyun, Raphael, Brian, Andreadis, Joanne, Johnson, Eric A.
Format:	Artigo
Sprog:	English
Udgivet:	American Society for Microbiology (ASM) 2008
Fag:	Evolutionary and Genomic Microbiology
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2394882/ https://ncbi.nlm.nih.gov/pubmed/18326685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02828-07
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!

id	pubmed-2394882
record_format	dspace
spelling	pubmed-23948822008-09-01 Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes Jacobson, Mark J. Lin, Guangyun Raphael, Brian Andreadis, Joanne Johnson, Eric A. Appl Environ Microbiol Evolutionary and Genomic Microbiology Neurotoxin cluster gene sequences and arrangements were elucidated for strains of Clostridium botulinum encoding botulinum neurotoxin (BoNT) subtypes A3, A4, and a unique A1-producing strain (HA(−) Orfx(+) A1). These sequences were compared to the known neurotoxin cluster sequences of C. botulinum strains that produce BoNT/A1 and BoNT/A2 and possess either a hemagglutinin (HA) or an Orfx cluster, respectively. The A3 and HA(−) Orfx(+) A1 strains demonstrated a neurotoxin cluster arrangement similar to that found in A2. The A4 strain analyzed possessed two sets of neurotoxin clusters that were similar to what has been found in the A(B) strains: an HA cluster associated with the BoNT/B gene and an Orfx cluster associated with the BoNT/A4 gene. The nucleotide and amino acid sequences of the neurotoxin cluster-specific genes were determined for each neurotoxin cluster and compared among strains. Additionally, the ntnh gene of each strain was compared on both the nucleotide and amino acid levels. The degree of similarity of the sequences of the ntnh genes and corresponding amino acid sequences correlated with the neurotoxin cluster type to which the ntnh gene was assigned. American Society for Microbiology (ASM) 2008-05 2008-03-07 /pmc/articles/PMC2394882/ /pubmed/18326685 http://dx.doi.org/10.1128/AEM.02828-07 Text en Copyright © 2008, American Society for Microbiology
institution	US National Library of Medicine
collection	PubMed Central
language	English
format	Article
topic	Evolutionary and Genomic Microbiology
spellingShingle	Evolutionary and Genomic Microbiology Jacobson, Mark J. Lin, Guangyun Raphael, Brian Andreadis, Joanne Johnson, Eric A. Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
description	Neurotoxin cluster gene sequences and arrangements were elucidated for strains of Clostridium botulinum encoding botulinum neurotoxin (BoNT) subtypes A3, A4, and a unique A1-producing strain (HA(−) Orfx(+) A1). These sequences were compared to the known neurotoxin cluster sequences of C. botulinum strains that produce BoNT/A1 and BoNT/A2 and possess either a hemagglutinin (HA) or an Orfx cluster, respectively. The A3 and HA(−) Orfx(+) A1 strains demonstrated a neurotoxin cluster arrangement similar to that found in A2. The A4 strain analyzed possessed two sets of neurotoxin clusters that were similar to what has been found in the A(B) strains: an HA cluster associated with the BoNT/B gene and an Orfx cluster associated with the BoNT/A4 gene. The nucleotide and amino acid sequences of the neurotoxin cluster-specific genes were determined for each neurotoxin cluster and compared among strains. Additionally, the ntnh gene of each strain was compared on both the nucleotide and amino acid levels. The degree of similarity of the sequences of the ntnh genes and corresponding amino acid sequences correlated with the neurotoxin cluster type to which the ntnh gene was assigned.
author	Jacobson, Mark J. Lin, Guangyun Raphael, Brian Andreadis, Joanne Johnson, Eric A.
author_facet	Jacobson, Mark J. Lin, Guangyun Raphael, Brian Andreadis, Joanne Johnson, Eric A.
author_sort	Jacobson, Mark J.
title	Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
title_short	Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
title_full	Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
title_fullStr	Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
title_full_unstemmed	Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes
title_sort	analysis of neurotoxin cluster genes in clostridium botulinum strains producing botulinum neurotoxin serotype a subtypes
publisher	American Society for Microbiology (ASM)
publisher_facet	American Society for Microbiology (ASM)
publishDate	2008
url	https://ncbi.nlm.nih.gov/pmc/articles/PMC2394882/ https://ncbi.nlm.nih.gov/pubmed/18326685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02828-07
_version_	1760584405266464768

Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes

Lignende værker