Functional and nonfunctional measles virus matrix genes from lethal human brain infections.

Subacute sclerosing panencephalitis (SSPE) is a lethal disease induced by the persistence of measles virus in the human brain. In many SSPE cases, the viral matrix (M) protein cannot be detected; in others, M proteins of the expected size are found and sequence analysis of M cDNAs has confirmed that...

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Main Authors: Ballart, I, Huber, M, Schmid, A, Cattaneo, R, Billeter, M A
Formato: Artigo
Idioma:en
Publicado em: 1991
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC240972/
https://ncbi.nlm.nih.gov/pubmed/2033668
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spelling pubmed-2409722003-12-01 Functional and nonfunctional measles virus matrix genes from lethal human brain infections. Ballart, I Huber, M Schmid, A Cattaneo, R Billeter, M A J Virol Research Article Subacute sclerosing panencephalitis (SSPE) is a lethal disease induced by the persistence of measles virus in the human brain. In many SSPE cases, the viral matrix (M) protein cannot be detected; in others, M proteins of the expected size are found and sequence analysis of M cDNAs has confirmed that the reading frames are intact, showing only several missense mutations. To determine whether these alterations result in nonfunctional proteins, we have replaced the M gene of an infectious full-length genomic cDNA (from vaccine strain Edmonston) with different M genes derived from four patients with SSPE. One of the SSPE M genes tested proved to be functionally competent, giving rise to a virus yielding titers similar to those of viruses containing the M gene from control lytic strains. The other three SSPE M genes were not functionally competent in the same test. In all three cases, the inactivating changes resided in the carboxyl-terminal half of the M protein, as shown by the exchange of either of the two genes halves. In summary, mutational M gene alterations, which either prevent synthesis of M protein altogether or only allow synthesis of nonfunctional M protein, have been detected by us and by others in 9 of 10 SSPE cases. The one functional M gene appears to be an exception to the rule, indicating that M gene alteration might not be an absolute requirement for disease development. 1991-06 /pmc/articles/PMC240972/ /pubmed/2033668 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Ballart, I
Huber, M
Schmid, A
Cattaneo, R
Billeter, M A
Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
description Subacute sclerosing panencephalitis (SSPE) is a lethal disease induced by the persistence of measles virus in the human brain. In many SSPE cases, the viral matrix (M) protein cannot be detected; in others, M proteins of the expected size are found and sequence analysis of M cDNAs has confirmed that the reading frames are intact, showing only several missense mutations. To determine whether these alterations result in nonfunctional proteins, we have replaced the M gene of an infectious full-length genomic cDNA (from vaccine strain Edmonston) with different M genes derived from four patients with SSPE. One of the SSPE M genes tested proved to be functionally competent, giving rise to a virus yielding titers similar to those of viruses containing the M gene from control lytic strains. The other three SSPE M genes were not functionally competent in the same test. In all three cases, the inactivating changes resided in the carboxyl-terminal half of the M protein, as shown by the exchange of either of the two genes halves. In summary, mutational M gene alterations, which either prevent synthesis of M protein altogether or only allow synthesis of nonfunctional M protein, have been detected by us and by others in 9 of 10 SSPE cases. The one functional M gene appears to be an exception to the rule, indicating that M gene alteration might not be an absolute requirement for disease development.
author Ballart, I
Huber, M
Schmid, A
Cattaneo, R
Billeter, M A
author_facet Ballart, I
Huber, M
Schmid, A
Cattaneo, R
Billeter, M A
author_sort Ballart, I
title Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
title_short Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
title_full Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
title_fullStr Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
title_full_unstemmed Functional and nonfunctional measles virus matrix genes from lethal human brain infections.
title_sort functional and nonfunctional measles virus matrix genes from lethal human brain infections.
publishDate 1991
url https://ncbi.nlm.nih.gov/pmc/articles/PMC240972/
https://ncbi.nlm.nih.gov/pubmed/2033668
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