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Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma
Pathogenetic mechanisms of hepatocellular carcinoma (HCC) are still unclear and new tools for diagnostic and therapeutic purposes are ongoing. We have assessed whether squamous cell carcinoma antigen (SCCA), a serpin overexpressed in neoplastic cells of epithelial origin, is also expressed in liver...
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Nature Publishing Group
2004
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2410161/ https://ncbi.nlm.nih.gov/pubmed/14970861 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.bjc.6601543 |
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pubmed-24101612009-09-10 Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma Pontisso, P Calabrese, F Benvegnù, L Lise, M Belluco, C Ruvoletto, M G De Falco, S Marino, M Valente, M Nitti, D Gatta, A Fassina, G Br J Cancer Molecular and Cellular Pathology Pathogenetic mechanisms of hepatocellular carcinoma (HCC) are still unclear and new tools for diagnostic and therapeutic purposes are ongoing. We have assessed whether squamous cell carcinoma antigen (SCCA), a serpin overexpressed in neoplastic cells of epithelial origin, is also expressed in liver cancer. Squamous cell carcinoma antigen was evaluated by immunohistochemistry in 65 HCCs of different aetiology and in 20 normal livers. Proliferative activity was assessed using MIB-1 antibody. In 18 surgical samples, tumour and nontumour liver tissue was available for SCCA cDNA amplification and sequencing. Squamous cell carcinoma antigen was detected in 55 out of 65 (85%) tumour specimens, but in none of the 20 controls. In the majority of the cases, the positive signal was found in the cytoplasm of more than 50% of the hepatocytes. Low or undetectable SCCA (score⩽1) was associated to lower MIB-1 labelling index, compared to cases with SCCA score ⩾2 (mean±s.d.: 2%±2.4 vs 7.5%±10.3, P<0.05). Squamous cell carcinoma antigen mRNA could be directly sequenced in 14 out of 18 liver tumours but in none of the corresponding nontumour samples. From sequence alignment, a novel SCCA1 variant (G(351) to A) was identified in five cases, while SCCA1 was revealed in six cases and SCCA2 in three cases. In conclusion, SCCA variants are overexpressed in HCC, independently of tumour aetiology. A novel SCCA1 variant has been identified in one third of liver tumours. Nature Publishing Group 2004-02-23 2004-02-17 /pmc/articles/PMC2410161/ /pubmed/14970861 http://dx.doi.org/10.1038/sj.bjc.6601543 Text en Copyright © 2004 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
English |
| format |
Article |
| topic |
Molecular and Cellular Pathology |
| spellingShingle |
Molecular and Cellular Pathology Pontisso, P Calabrese, F Benvegnù, L Lise, M Belluco, C Ruvoletto, M G De Falco, S Marino, M Valente, M Nitti, D Gatta, A Fassina, G Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| description |
Pathogenetic mechanisms of hepatocellular carcinoma (HCC) are still unclear and new tools for diagnostic and therapeutic purposes are ongoing. We have assessed whether squamous cell carcinoma antigen (SCCA), a serpin overexpressed in neoplastic cells of epithelial origin, is also expressed in liver cancer. Squamous cell carcinoma antigen was evaluated by immunohistochemistry in 65 HCCs of different aetiology and in 20 normal livers. Proliferative activity was assessed using MIB-1 antibody. In 18 surgical samples, tumour and nontumour liver tissue was available for SCCA cDNA amplification and sequencing. Squamous cell carcinoma antigen was detected in 55 out of 65 (85%) tumour specimens, but in none of the 20 controls. In the majority of the cases, the positive signal was found in the cytoplasm of more than 50% of the hepatocytes. Low or undetectable SCCA (score⩽1) was associated to lower MIB-1 labelling index, compared to cases with SCCA score ⩾2 (mean±s.d.: 2%±2.4 vs 7.5%±10.3, P<0.05). Squamous cell carcinoma antigen mRNA could be directly sequenced in 14 out of 18 liver tumours but in none of the corresponding nontumour samples. From sequence alignment, a novel SCCA1 variant (G(351) to A) was identified in five cases, while SCCA1 was revealed in six cases and SCCA2 in three cases. In conclusion, SCCA variants are overexpressed in HCC, independently of tumour aetiology. A novel SCCA1 variant has been identified in one third of liver tumours. |
| author |
Pontisso, P Calabrese, F Benvegnù, L Lise, M Belluco, C Ruvoletto, M G De Falco, S Marino, M Valente, M Nitti, D Gatta, A Fassina, G |
| author_facet |
Pontisso, P Calabrese, F Benvegnù, L Lise, M Belluco, C Ruvoletto, M G De Falco, S Marino, M Valente, M Nitti, D Gatta, A Fassina, G |
| author_sort |
Pontisso, P |
| title |
Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| title_short |
Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| title_full |
Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| title_fullStr |
Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| title_full_unstemmed |
Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| title_sort |
overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma |
| publisher |
Nature Publishing Group |
| publisher_facet |
Nature Publishing Group |
| publishDate |
2004 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC2410161/ https://ncbi.nlm.nih.gov/pubmed/14970861 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.bjc.6601543 |
| _version_ |
1760619424807649280 |