Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli

Deoxyribonucleic acid (DNA) of Escherichia coli was found to be attached to the cell membrane at about 20 points. This was determined by fractionation of X-irradiated cells with the M band (magnesium-Sarkosyl crystals) technique. The number of attachment points was computed from the relationship bet...

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Manylion Llyfryddiaeth
Prif Awduron: Dworsky, P., Schaechter, M.
Fformat: Erthygl
Iaith:en
Cyhoeddwyd: 1973
Pynciau:
Morphology and Ultrastructure
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC246496/
https://ncbi.nlm.nih.gov/pubmed/4584813
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spelling pubmed-2464962003-12-16 Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli Dworsky, P. Schaechter, M. J Bacteriol Morphology and Ultrastructure Deoxyribonucleic acid (DNA) of Escherichia coli was found to be attached to the cell membrane at about 20 points. This was determined by fractionation of X-irradiated cells with the M band (magnesium-Sarkosyl crystals) technique. The number of attachment points was computed from the relationship between the amount of DNA in M bands and the number of double-strand breaks introduced by the X-ray treatment. The number of attachment points was decreased fourfold by treatment of cells with rifampin. This effect was apparently due to the action of the drug on ribonucleic acid (RNA) polymerase since the drug did not affect a mutant whose RNA polymerase is resistant to rifampin. This suggests that there may be two classes of attachment points of DNA on the membrane, some of which are removed by rifampin treatment and some which are not. Rifampin treatment also resulted in the uncondensing of isolated nucleoids and in an axial appearance of the nucleoids in ultrathin sections. The results suggest that RNA polymerase plays a role, direct or indirect, in maintaining the structure of the bacterial nucleoid and in some of its attachment to the membrane. 1973-12 /pmc/articles/PMC246496/ /pubmed/4584813 Text en Copyright © 1973 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Morphology and Ultrastructure
spellingShingle Morphology and Ultrastructure
Dworsky, P.
Schaechter, M.
Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
description Deoxyribonucleic acid (DNA) of Escherichia coli was found to be attached to the cell membrane at about 20 points. This was determined by fractionation of X-irradiated cells with the M band (magnesium-Sarkosyl crystals) technique. The number of attachment points was computed from the relationship between the amount of DNA in M bands and the number of double-strand breaks introduced by the X-ray treatment. The number of attachment points was decreased fourfold by treatment of cells with rifampin. This effect was apparently due to the action of the drug on ribonucleic acid (RNA) polymerase since the drug did not affect a mutant whose RNA polymerase is resistant to rifampin. This suggests that there may be two classes of attachment points of DNA on the membrane, some of which are removed by rifampin treatment and some which are not. Rifampin treatment also resulted in the uncondensing of isolated nucleoids and in an axial appearance of the nucleoids in ultrathin sections. The results suggest that RNA polymerase plays a role, direct or indirect, in maintaining the structure of the bacterial nucleoid and in some of its attachment to the membrane.
author Dworsky, P.
Schaechter, M.
author_facet Dworsky, P.
Schaechter, M.
author_sort Dworsky, P.
title Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
title_short Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
title_full Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
title_fullStr Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
title_full_unstemmed Effect of Rifampin on the Structure and Membrane Attachment of the Nucleoid of Escherichia coli
title_sort effect of rifampin on the structure and membrane attachment of the nucleoid of escherichia coli
publishDate 1973
url https://ncbi.nlm.nih.gov/pmc/articles/PMC246496/
https://ncbi.nlm.nih.gov/pubmed/4584813
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