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Mannitol Uptake by Saccharomyces cerevisiae
The uptake of mannitol, a nonmetabolized hexitol, by Saccharomyces cerevisiae was measured. Various characteristics examined include: effects of temperature on uptake, inhibition of uptake by uranyl nitrate, competition for uptake by glucose, counterflow of mannitol by glucose, and the affinity of m...
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| Materialtyp: | Artikel |
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1971
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC248497/ https://ncbi.nlm.nih.gov/pubmed/5547989 |
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pubmed-2484972003-12-16 Mannitol Uptake by Saccharomyces cerevisiae Maxwell, W. A. Spoerl, Edward J Bacteriol Physiology and Metabolism The uptake of mannitol, a nonmetabolized hexitol, by Saccharomyces cerevisiae was measured. Various characteristics examined include: effects of temperature on uptake, inhibition of uptake by uranyl nitrate, competition for uptake by glucose, counterflow of mannitol by glucose, and the affinity of mannitol for a carrier system as measured by a Michaelis constant. That energy is required for uptake was shown by a decreased uptake in the presence of energy inhibitors, by an increased uptake upon addition of energy sources, and by the absence of uptake under anaerobic conditions with no fermentable energy sources available. That mannitol is bound to some cellular constituent after it enters the cell was shown by its attachment to non-dialyzable cell fragments and by the lack of an osmotic response, both of which are consistent with a minimal efflux. 1971-03 /pmc/articles/PMC248497/ /pubmed/5547989 Text en Copyright © 1971 American Society for Microbiology |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Physiology and Metabolism |
| spellingShingle |
Physiology and Metabolism Maxwell, W. A. Spoerl, Edward Mannitol Uptake by Saccharomyces cerevisiae |
| description |
The uptake of mannitol, a nonmetabolized hexitol, by Saccharomyces cerevisiae was measured. Various characteristics examined include: effects of temperature on uptake, inhibition of uptake by uranyl nitrate, competition for uptake by glucose, counterflow of mannitol by glucose, and the affinity of mannitol for a carrier system as measured by a Michaelis constant. That energy is required for uptake was shown by a decreased uptake in the presence of energy inhibitors, by an increased uptake upon addition of energy sources, and by the absence of uptake under anaerobic conditions with no fermentable energy sources available. That mannitol is bound to some cellular constituent after it enters the cell was shown by its attachment to non-dialyzable cell fragments and by the lack of an osmotic response, both of which are consistent with a minimal efflux. |
| author |
Maxwell, W. A. Spoerl, Edward |
| author_facet |
Maxwell, W. A. Spoerl, Edward |
| author_sort |
Maxwell, W. A. |
| title |
Mannitol Uptake by Saccharomyces cerevisiae |
| title_short |
Mannitol Uptake by Saccharomyces cerevisiae |
| title_full |
Mannitol Uptake by Saccharomyces cerevisiae |
| title_fullStr |
Mannitol Uptake by Saccharomyces cerevisiae |
| title_full_unstemmed |
Mannitol Uptake by Saccharomyces cerevisiae |
| title_sort |
mannitol uptake by saccharomyces cerevisiae |
| publishDate |
1971 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC248497/ https://ncbi.nlm.nih.gov/pubmed/5547989 |
| _version_ |
1759048993597292544 |