Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome

Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing protein...

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Autors principals: Albee, Alison J., Wiese, Christiane
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Idioma:English
Publicat: The American Society for Cell Biology 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2488304/
https://ncbi.nlm.nih.gov/pubmed/18508920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E07-11-1204
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spelling pubmed-24883042008-09-28 Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome Albee, Alison J. Wiese, Christiane Mol Biol Cell Articles Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor overexpressed gene (TOG) family to the centrosome and to facilitate their loading onto newly emerging microtubules. Using Xenopus egg extracts and in vitro assays, we show that the Xenopus TACC protein maskin is required for centrosome function beyond recruiting the Xenopus TOG protein XMAP215. The conserved C-terminal TACC domain of maskin is both necessary and sufficient to restore centrosome function in maskin-depleted extracts, and we provide evidence that the N terminus of maskin inhibits the function of the TACC domain. Time-lapse video microscopy reveals that microtubule dynamics in Xenopus egg extracts are unaffected by maskin depletion. Our results provide direct experimental evidence of a role for maskin in centrosome function and suggest that maskin is required for microtubule anchoring at the centrosome. The American Society for Cell Biology 2008-08 /pmc/articles/PMC2488304/ /pubmed/18508920 http://dx.doi.org/10.1091/mbc.E07-11-1204 Text en © 2008 by The American Society for Cell Biology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Articles
spellingShingle Articles
Albee, Alison J.
Wiese, Christiane
Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
description Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor overexpressed gene (TOG) family to the centrosome and to facilitate their loading onto newly emerging microtubules. Using Xenopus egg extracts and in vitro assays, we show that the Xenopus TACC protein maskin is required for centrosome function beyond recruiting the Xenopus TOG protein XMAP215. The conserved C-terminal TACC domain of maskin is both necessary and sufficient to restore centrosome function in maskin-depleted extracts, and we provide evidence that the N terminus of maskin inhibits the function of the TACC domain. Time-lapse video microscopy reveals that microtubule dynamics in Xenopus egg extracts are unaffected by maskin depletion. Our results provide direct experimental evidence of a role for maskin in centrosome function and suggest that maskin is required for microtubule anchoring at the centrosome.
author Albee, Alison J.
Wiese, Christiane
author_facet Albee, Alison J.
Wiese, Christiane
author_sort Albee, Alison J.
title Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
title_short Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
title_full Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
title_fullStr Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
title_full_unstemmed Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome
title_sort xenopus tacc3/maskin is not required for microtubule stability but is required for anchoring microtubules at the centrosome
publisher The American Society for Cell Biology
publisher_facet The American Society for Cell Biology
publishDate 2008
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2488304/
https://ncbi.nlm.nih.gov/pubmed/18508920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E07-11-1204
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