Primary Human Hepatocytes Are Susceptible to Infection by Hepatitis Delta Virus Assembled with Envelope Proteins of Woodchuck Hepatitis Virus

Hepatitis B virus (HBV) and hepatitis delta virus (HDV) share the HBV envelope proteins. When woodchucks chronically infected with woodchuck hepatitis virus (WHV) are superinfected with HDV, they produce HDV with a WHV envelope, wHDV. Several lines of evidence are provided that wHDV infects not only...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Gudima, Severin, He, Yiping, Chai, Ning, Bruss, Volker, Urban, Stephan, Mason, William, Taylor, John
Fformat: Erthygl
Iaith:English
Cyhoeddwyd: American Society for Microbiology (ASM) 2008
Pynciau:
Virus-Cell Interactions
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2493318/
https://ncbi.nlm.nih.gov/pubmed/18495772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00576-08
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Crynodeb:Hepatitis B virus (HBV) and hepatitis delta virus (HDV) share the HBV envelope proteins. When woodchucks chronically infected with woodchuck hepatitis virus (WHV) are superinfected with HDV, they produce HDV with a WHV envelope, wHDV. Several lines of evidence are provided that wHDV infects not only cultured primary woodchuck hepatocytes (PWH) but also primary human hepatocytes (PHH). Surprisingly, HBV-enveloped HDV (hHDV) and wHDV infected PHH with comparable efficiencies; however, hHDV did not infect PWH. The basis for these host range specificities was investigated using as inhibitors peptides bearing species-specific pre-S (where S is the small envelope protein) sequences. It was found that pre-S1 contributed to the ability of wHDV to infect both PHH and PWH. In addition, the inability of hHDV to infect PWH was not overcome using a chimeric form of hHDV containing WHV S protein, again supporting the essential role of pre-S1 in infection of target cells. One interpretation of these data is that host range specificity of HDV is determined entirely by pre-S1 and that the WHV and HBV pre-S1 proteins recognize different receptors on PHH.

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