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2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii
The oxidation of ornithine in the presence of proline by crude extracts of Clostridium sticklandii cells was stimulated by nicotinamide adenine dinucleotide, coenzyme A, α-ketoglutarate, dimethylbenzimidazolyl cobamide (DBC) coenzyme, MgCl(2), and adenosine diphosphate. Deletion of various cofactors...
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pubmed-2504532003-12-16 2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii Dyer, John K. Costilow, Ralph N. J Bacteriol Physiology and Metabolism The oxidation of ornithine in the presence of proline by crude extracts of Clostridium sticklandii cells was stimulated by nicotinamide adenine dinucleotide, coenzyme A, α-ketoglutarate, dimethylbenzimidazolyl cobamide (DBC) coenzyme, MgCl(2), and adenosine diphosphate. Deletion of various cofactors resulted in the accumulation of a new dibasic amino acid which was identified as 2,4-diaminovaleric acid. Both the oxidation of ornithine to alanine and acetate and the conversion of ornithine to 2,4-diaminovaleric acid were stimulated by addition of DBC coenzyme, and both were inhibited by intrinsic factor, an inhibitor of cobamide coenzyme-dependent reactions. This inhibition was reversed by addition of DBC coenzyme. However, the oxidation of 2,4-diaminovaleric acid was insensitive to added intrinsic factor. The data indicate that 2,4-diaminovaleric acid represents the first intermediate in the oxidation of ornithine by C. sticklandii. 1970-01 /pmc/articles/PMC250453/ /pubmed/4312545 Text en Copyright © 1970 American Society for Microbiology |
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US National Library of Medicine |
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PubMed Central |
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Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Genetics and Molecular Biology Enzymology Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Enzymology Genetics and Molecular Biology Genetics and Molecular Biology Enzymology Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Enzymology Enzymology Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Enzymology Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Genetics and Molecular Biology Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article |
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Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Genetics and Molecular Biology Enzymology Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Enzymology Genetics and Molecular Biology Genetics and Molecular Biology Enzymology Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Enzymology Enzymology Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Enzymology Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Morphology and Ultrastructure Physiology and Metabolism Physiology and Metabolism Physiology and Metabolism Genetics and Molecular Biology Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Dyer, John K. Costilow, Ralph N. Rizza, Victor Tucker, Anne N. White, David C. Ou, Li-Tse Marquis, Robert E. Nose, K. Ono, M. Mizuno, D. Fjellstedt, Tho̊rsten A. Ogur, Maurice Webster, Francis H. Lechowich, Richard V. Mallavia, Louis P. Weiss, Emilio Weiss, Emilio Michaud, R. N. Delwiche, E. A. Michaud, R. N. Carrow, J. A. Delwiche, E. A. Daron, Harlow H. Harold, F. M. Baarda, J. R. Pavlasova, E. Wisnieski, Bernadine J. Keith, Alec D. Resnick, Michael R. Barlati, Sergio Ciferri, Orio Imsande, John Downer, D. N. Davis, W. B. Byers, B. R. Previc, Edward Fister, George Matz, L. L. Beaman, T. Cabrera Gerhardt, Philipp Held, William A. Smith, Oliver H. Held, William A. Smith, Oliver H. Lindström, E. Börje Boman, Hans G. Steele, Barbara B. Lindqvist, R. Christer Nordström, Kurt Snellen, James E. Raj, H. D. Abram, Dinah Mitchen, J. R. Koffler, Henry Vatter, A. E. Boatman, Edwin S. Kenny, George E. Matsumoto, A. Manire, G. P. Oppenheim, Joel Marcus, Leon Oppenheim, Joel Fisher, Robert J. Wilson, P. W. Marcus, Leon Nanninga, N. Bayer, M. E. Remsen, C. C. Matheson, Audria Donaldson, David M. Wildermuth, H. Weinberger, Steven Gilvarg, Charles Reichenbach, Hans Dworkin, Martin Vary, James C. Kornberg, Arthur Barlati, Sergio Prehaud, C Coulon, P LaFay, F Thiers, C Flamand, A Charley, B Laude, H Mettenleiter, T C Lomniczi, B Sugg, N Schreurs, C Ben-Porat, T Ochiai, H Kurokawa, M Hayashi, K Niwayama, S Raju, R Kolakofsky, D Tollefson, A E Wold, W S Ikuta, K Luftig, R B Tsiang, M Weiss, B G Schlesinger, S Parks, C L Banerjee, S Spector, D J Trempe, J P Carter, B J Bova, C A Olsen, J C Swanstrom, R Crum, C P Nuovo, G Friedman, D Silverstein, S J Carmichael, E P Kosovsky, M J Weller, S K Fujinami, R S Nelson, J A Walker, L Oldstone, M B 2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| description |
The oxidation of ornithine in the presence of proline by crude extracts of Clostridium sticklandii cells was stimulated by nicotinamide adenine dinucleotide, coenzyme A, α-ketoglutarate, dimethylbenzimidazolyl cobamide (DBC) coenzyme, MgCl(2), and adenosine diphosphate. Deletion of various cofactors resulted in the accumulation of a new dibasic amino acid which was identified as 2,4-diaminovaleric acid. Both the oxidation of ornithine to alanine and acetate and the conversion of ornithine to 2,4-diaminovaleric acid were stimulated by addition of DBC coenzyme, and both were inhibited by intrinsic factor, an inhibitor of cobamide coenzyme-dependent reactions. This inhibition was reversed by addition of DBC coenzyme. However, the oxidation of 2,4-diaminovaleric acid was insensitive to added intrinsic factor. The data indicate that 2,4-diaminovaleric acid represents the first intermediate in the oxidation of ornithine by C. sticklandii. |
| author |
Dyer, John K. Costilow, Ralph N. Rizza, Victor Tucker, Anne N. White, David C. Ou, Li-Tse Marquis, Robert E. Nose, K. Ono, M. Mizuno, D. Fjellstedt, Tho̊rsten A. Ogur, Maurice Webster, Francis H. Lechowich, Richard V. Mallavia, Louis P. Weiss, Emilio Weiss, Emilio Michaud, R. N. Delwiche, E. A. Michaud, R. N. Carrow, J. A. Delwiche, E. A. Daron, Harlow H. Harold, F. M. Baarda, J. R. Pavlasova, E. Wisnieski, Bernadine J. Keith, Alec D. Resnick, Michael R. Barlati, Sergio Ciferri, Orio Imsande, John Downer, D. N. Davis, W. B. Byers, B. R. Previc, Edward Fister, George Matz, L. L. Beaman, T. Cabrera Gerhardt, Philipp Held, William A. Smith, Oliver H. Held, William A. Smith, Oliver H. Lindström, E. Börje Boman, Hans G. Steele, Barbara B. Lindqvist, R. Christer Nordström, Kurt Snellen, James E. Raj, H. D. Abram, Dinah Mitchen, J. R. Koffler, Henry Vatter, A. E. Boatman, Edwin S. Kenny, George E. Matsumoto, A. Manire, G. P. Oppenheim, Joel Marcus, Leon Oppenheim, Joel Fisher, Robert J. Wilson, P. W. Marcus, Leon Nanninga, N. Bayer, M. E. Remsen, C. C. Matheson, Audria Donaldson, David M. Wildermuth, H. Weinberger, Steven Gilvarg, Charles Reichenbach, Hans Dworkin, Martin Vary, James C. Kornberg, Arthur Barlati, Sergio Prehaud, C Coulon, P LaFay, F Thiers, C Flamand, A Charley, B Laude, H Mettenleiter, T C Lomniczi, B Sugg, N Schreurs, C Ben-Porat, T Ochiai, H Kurokawa, M Hayashi, K Niwayama, S Raju, R Kolakofsky, D Tollefson, A E Wold, W S Ikuta, K Luftig, R B Tsiang, M Weiss, B G Schlesinger, S Parks, C L Banerjee, S Spector, D J Trempe, J P Carter, B J Bova, C A Olsen, J C Swanstrom, R Crum, C P Nuovo, G Friedman, D Silverstein, S J Carmichael, E P Kosovsky, M J Weller, S K Fujinami, R S Nelson, J A Walker, L Oldstone, M B |
| author_facet |
Dyer, John K. Costilow, Ralph N. Rizza, Victor Tucker, Anne N. White, David C. Ou, Li-Tse Marquis, Robert E. Nose, K. Ono, M. Mizuno, D. Fjellstedt, Tho̊rsten A. Ogur, Maurice Webster, Francis H. Lechowich, Richard V. Mallavia, Louis P. Weiss, Emilio Weiss, Emilio Michaud, R. N. Delwiche, E. A. Michaud, R. N. Carrow, J. A. Delwiche, E. A. Daron, Harlow H. Harold, F. M. Baarda, J. R. Pavlasova, E. Wisnieski, Bernadine J. Keith, Alec D. Resnick, Michael R. Barlati, Sergio Ciferri, Orio Imsande, John Downer, D. N. Davis, W. B. Byers, B. R. Previc, Edward Fister, George Matz, L. L. Beaman, T. Cabrera Gerhardt, Philipp Held, William A. Smith, Oliver H. Held, William A. Smith, Oliver H. Lindström, E. Börje Boman, Hans G. Steele, Barbara B. Lindqvist, R. Christer Nordström, Kurt Snellen, James E. Raj, H. D. Abram, Dinah Mitchen, J. R. Koffler, Henry Vatter, A. E. Boatman, Edwin S. Kenny, George E. Matsumoto, A. Manire, G. P. Oppenheim, Joel Marcus, Leon Oppenheim, Joel Fisher, Robert J. Wilson, P. W. Marcus, Leon Nanninga, N. Bayer, M. E. Remsen, C. C. Matheson, Audria Donaldson, David M. Wildermuth, H. Weinberger, Steven Gilvarg, Charles Reichenbach, Hans Dworkin, Martin Vary, James C. Kornberg, Arthur Barlati, Sergio Prehaud, C Coulon, P LaFay, F Thiers, C Flamand, A Charley, B Laude, H Mettenleiter, T C Lomniczi, B Sugg, N Schreurs, C Ben-Porat, T Ochiai, H Kurokawa, M Hayashi, K Niwayama, S Raju, R Kolakofsky, D Tollefson, A E Wold, W S Ikuta, K Luftig, R B Tsiang, M Weiss, B G Schlesinger, S Parks, C L Banerjee, S Spector, D J Trempe, J P Carter, B J Bova, C A Olsen, J C Swanstrom, R Crum, C P Nuovo, G Friedman, D Silverstein, S J Carmichael, E P Kosovsky, M J Weller, S K Fujinami, R S Nelson, J A Walker, L Oldstone, M B |
| author_sort |
Dyer, John K. |
| title |
2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| title_short |
2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| title_full |
2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| title_fullStr |
2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| title_full_unstemmed |
2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii |
| title_sort |
2,4-diaminovaleric acid: an intermediate in the anaerobic oxidation of ornithine by clostridium sticklandii |
| publishDate |
1970 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC250453/ https://ncbi.nlm.nih.gov/pubmed/4312545 https://ncbi.nlm.nih.gov/pmc/articles/PMC250454/ https://ncbi.nlm.nih.gov/pubmed/5411759 https://ncbi.nlm.nih.gov/pmc/articles/PMC250455/ https://ncbi.nlm.nih.gov/pubmed/5411760 https://ncbi.nlm.nih.gov/pmc/articles/PMC250456/ https://ncbi.nlm.nih.gov/pubmed/4983641 https://ncbi.nlm.nih.gov/pmc/articles/PMC250457/ https://ncbi.nlm.nih.gov/pubmed/5411748 https://ncbi.nlm.nih.gov/pmc/articles/PMC250458/ https://ncbi.nlm.nih.gov/pubmed/4983642 https://ncbi.nlm.nih.gov/pmc/articles/PMC250459/ https://ncbi.nlm.nih.gov/pubmed/4983643 https://ncbi.nlm.nih.gov/pmc/articles/PMC250460/ https://ncbi.nlm.nih.gov/pubmed/16559074 https://ncbi.nlm.nih.gov/pmc/articles/PMC250461/ https://ncbi.nlm.nih.gov/pubmed/5460841 https://ncbi.nlm.nih.gov/pmc/articles/PMC250462/ https://ncbi.nlm.nih.gov/pubmed/4904232 https://ncbi.nlm.nih.gov/pmc/articles/PMC250463/ https://ncbi.nlm.nih.gov/pubmed/5411749 https://ncbi.nlm.nih.gov/pmc/articles/PMC250464/ https://ncbi.nlm.nih.gov/pubmed/4983644 https://ncbi.nlm.nih.gov/pmc/articles/PMC250465/ https://ncbi.nlm.nih.gov/pubmed/5411750 https://ncbi.nlm.nih.gov/pmc/articles/PMC250466/ https://ncbi.nlm.nih.gov/pubmed/4983645 https://ncbi.nlm.nih.gov/pmc/articles/PMC250467/ https://ncbi.nlm.nih.gov/pubmed/4983646 https://ncbi.nlm.nih.gov/pmc/articles/PMC250468/ https://ncbi.nlm.nih.gov/pubmed/4983647 https://ncbi.nlm.nih.gov/pmc/articles/PMC250469/ https://ncbi.nlm.nih.gov/pubmed/4904233 https://ncbi.nlm.nih.gov/pmc/articles/PMC250470/ https://ncbi.nlm.nih.gov/pubmed/4983648 https://ncbi.nlm.nih.gov/pmc/articles/PMC250471/ https://ncbi.nlm.nih.gov/pubmed/4904234 https://ncbi.nlm.nih.gov/pmc/articles/PMC250472/ https://ncbi.nlm.nih.gov/pubmed/4904235 https://ncbi.nlm.nih.gov/pmc/articles/PMC250473/ https://ncbi.nlm.nih.gov/pubmed/4983650 https://ncbi.nlm.nih.gov/pmc/articles/PMC250474/ https://ncbi.nlm.nih.gov/pubmed/4904236 https://ncbi.nlm.nih.gov/pmc/articles/PMC250475/ https://ncbi.nlm.nih.gov/pubmed/5411751 https://ncbi.nlm.nih.gov/pmc/articles/PMC250476/ https://ncbi.nlm.nih.gov/pubmed/4189228 https://ncbi.nlm.nih.gov/pmc/articles/PMC250477/ https://ncbi.nlm.nih.gov/pubmed/5411752 https://ncbi.nlm.nih.gov/pmc/articles/PMC250478/ https://ncbi.nlm.nih.gov/pubmed/5413965 https://ncbi.nlm.nih.gov/pmc/articles/PMC250479/ https://ncbi.nlm.nih.gov/pubmed/5411753 https://ncbi.nlm.nih.gov/pmc/articles/PMC250480/ https://ncbi.nlm.nih.gov/pubmed/4312544 https://ncbi.nlm.nih.gov/pmc/articles/PMC250481/ https://ncbi.nlm.nih.gov/pubmed/4904237 https://ncbi.nlm.nih.gov/pmc/articles/PMC250482/ https://ncbi.nlm.nih.gov/pubmed/4189229 https://ncbi.nlm.nih.gov/pmc/articles/PMC250483/ https://ncbi.nlm.nih.gov/pubmed/4983652 https://ncbi.nlm.nih.gov/pmc/articles/PMC250484/ https://ncbi.nlm.nih.gov/pubmed/4189230 https://ncbi.nlm.nih.gov/pmc/articles/PMC250485/ https://ncbi.nlm.nih.gov/pubmed/5411754 https://ncbi.nlm.nih.gov/pmc/articles/PMC250486/ https://ncbi.nlm.nih.gov/pubmed/5411755 https://ncbi.nlm.nih.gov/pmc/articles/PMC250487/ https://ncbi.nlm.nih.gov/pubmed/4983653 https://ncbi.nlm.nih.gov/pmc/articles/PMC250488/ https://ncbi.nlm.nih.gov/pubmed/4983654 https://ncbi.nlm.nih.gov/pmc/articles/PMC250493/ https://ncbi.nlm.nih.gov/pubmed/2446011 https://ncbi.nlm.nih.gov/pmc/articles/PMC250494/ https://ncbi.nlm.nih.gov/pubmed/2824858 https://ncbi.nlm.nih.gov/pmc/articles/PMC250495/ https://ncbi.nlm.nih.gov/pubmed/2824839 https://ncbi.nlm.nih.gov/pmc/articles/PMC250496/ https://ncbi.nlm.nih.gov/pubmed/3334744 https://ncbi.nlm.nih.gov/pmc/articles/PMC250497/ https://ncbi.nlm.nih.gov/pubmed/3334745 https://ncbi.nlm.nih.gov/pmc/articles/PMC250498/ https://ncbi.nlm.nih.gov/pubmed/3275435 https://ncbi.nlm.nih.gov/pmc/articles/PMC250499/ https://ncbi.nlm.nih.gov/pubmed/3334749 https://ncbi.nlm.nih.gov/pmc/articles/PMC250500/ https://ncbi.nlm.nih.gov/pubmed/3334750 https://ncbi.nlm.nih.gov/pmc/articles/PMC250501/ https://ncbi.nlm.nih.gov/pubmed/3334751 https://ncbi.nlm.nih.gov/pmc/articles/PMC250502/ https://ncbi.nlm.nih.gov/pubmed/2824856 https://ncbi.nlm.nih.gov/pmc/articles/PMC250503/ https://ncbi.nlm.nih.gov/pubmed/2824857 https://ncbi.nlm.nih.gov/pmc/articles/PMC250504/ https://ncbi.nlm.nih.gov/pubmed/2824859 https://ncbi.nlm.nih.gov/pmc/articles/PMC250505/ https://ncbi.nlm.nih.gov/pubmed/2824860 https://ncbi.nlm.nih.gov/pmc/articles/PMC250506/ https://ncbi.nlm.nih.gov/pubmed/2446012 |
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