Origin and Sequence of Chromosome Replication in Escherichia coli B/r

The initial rates of induced synthesis of tryptophanase, β-galactosidase, and d-serine deaminase were measured in relation to the chromosome replication cycle of Escherichia coli B/r. Exponentially growing cultures were exposed briefly to (14)C-thymidine or the appropriate inducers (or both), and th...

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Autor principal: Helmstetter, Charles E.
Formato: Artigo
Idioma:en
Publicado em: 1968
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC252188/
https://ncbi.nlm.nih.gov/pubmed/4870279
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spelling pubmed-2521882004-01-27 Origin and Sequence of Chromosome Replication in Escherichia coli B/r Helmstetter, Charles E. J Bacteriol Genetics and Molecular Biology The initial rates of induced synthesis of tryptophanase, β-galactosidase, and d-serine deaminase were measured in relation to the chromosome replication cycle of Escherichia coli B/r. Exponentially growing cultures were exposed briefly to (14)C-thymidine or the appropriate inducers (or both), and the amount of label or enzyme (or both) in cells of different ages was found by measuring these quantities in their progeny. The rates of induced synthesis of the three enzymes increased abruptly at about 4, 20, and 34 min, respectively, after the start of a round of replication lasting 40 min. By matching this sequence to the ind, lac, and Dsd loci on the genetic map of E. coli K-12, it was estimated that replication began at about 8 o'clock (60 min) and proceeded clockwise. In rapidly growing cells, the sequence during the division cycle was consistent with the concept that rounds of replication overlapped. 1968-05 /pmc/articles/PMC252188/ /pubmed/4870279 Text en Copyright © 1968 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Genetics and Molecular Biology
spellingShingle Genetics and Molecular Biology
Helmstetter, Charles E.
Origin and Sequence of Chromosome Replication in Escherichia coli B/r
description The initial rates of induced synthesis of tryptophanase, β-galactosidase, and d-serine deaminase were measured in relation to the chromosome replication cycle of Escherichia coli B/r. Exponentially growing cultures were exposed briefly to (14)C-thymidine or the appropriate inducers (or both), and the amount of label or enzyme (or both) in cells of different ages was found by measuring these quantities in their progeny. The rates of induced synthesis of the three enzymes increased abruptly at about 4, 20, and 34 min, respectively, after the start of a round of replication lasting 40 min. By matching this sequence to the ind, lac, and Dsd loci on the genetic map of E. coli K-12, it was estimated that replication began at about 8 o'clock (60 min) and proceeded clockwise. In rapidly growing cells, the sequence during the division cycle was consistent with the concept that rounds of replication overlapped.
author Helmstetter, Charles E.
author_facet Helmstetter, Charles E.
author_sort Helmstetter, Charles E.
title Origin and Sequence of Chromosome Replication in Escherichia coli B/r
title_short Origin and Sequence of Chromosome Replication in Escherichia coli B/r
title_full Origin and Sequence of Chromosome Replication in Escherichia coli B/r
title_fullStr Origin and Sequence of Chromosome Replication in Escherichia coli B/r
title_full_unstemmed Origin and Sequence of Chromosome Replication in Escherichia coli B/r
title_sort origin and sequence of chromosome replication in escherichia coli b/r
publishDate 1968
url https://ncbi.nlm.nih.gov/pmc/articles/PMC252188/
https://ncbi.nlm.nih.gov/pubmed/4870279
_version_ 1759049501648093184

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