Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa

Nitrite was formed from hydroxylamine and several oximes by intact cells and extracts of Pseudomonas aeruginosa. The activity was induced by the presence of oximes in the culture medium. Nitroalkanes were not intermediates in the conversion of acetaldoxime, acetone oxime, or butanone oxime to nitrit...

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Main Authors: Amarger, Noelle, Alexander, M.
Formato: Artigo
Idioma:en
Publicado em: 1968
Assuntos:
Microbial Physiology and Metabolism
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC252191/
https://ncbi.nlm.nih.gov/pubmed/4384968
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spelling pubmed-2521912004-01-27 Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa Amarger, Noelle Alexander, M. J Bacteriol Microbial Physiology and Metabolism Nitrite was formed from hydroxylamine and several oximes by intact cells and extracts of Pseudomonas aeruginosa. The activity was induced by the presence of oximes in the culture medium. Nitroalkanes were not intermediates in the conversion of acetaldoxime, acetone oxime, or butanone oxime to nitrite, since nitromethane inhibited the formation of nitrite from the nitro compounds but not from the corresponding oximes. The oxime apparently functions as a constant source of hydroxylamine during growth of the bacterium. Hydroxylamine at low concentration was converted stoichiometrically to nitrite by extracts of the bacterium; high concentrations were inhibitory. Nicotinamide adenine dinucleotide phosphate, oxygen, and other unidentified cofactors were necessary for the reaction. Actively nitrifying extracts possessed no hydroxylamine-cytochrome c reductase activity. Hyponitrite, nitrous oxide, and nitric oxide were not metabolized. 1968-05 /pmc/articles/PMC252191/ /pubmed/4384968 Text en Copyright © 1968 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Microbial Physiology and Metabolism
spellingShingle Microbial Physiology and Metabolism
Amarger, Noelle
Alexander, M.
Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
description Nitrite was formed from hydroxylamine and several oximes by intact cells and extracts of Pseudomonas aeruginosa. The activity was induced by the presence of oximes in the culture medium. Nitroalkanes were not intermediates in the conversion of acetaldoxime, acetone oxime, or butanone oxime to nitrite, since nitromethane inhibited the formation of nitrite from the nitro compounds but not from the corresponding oximes. The oxime apparently functions as a constant source of hydroxylamine during growth of the bacterium. Hydroxylamine at low concentration was converted stoichiometrically to nitrite by extracts of the bacterium; high concentrations were inhibitory. Nicotinamide adenine dinucleotide phosphate, oxygen, and other unidentified cofactors were necessary for the reaction. Actively nitrifying extracts possessed no hydroxylamine-cytochrome c reductase activity. Hyponitrite, nitrous oxide, and nitric oxide were not metabolized.
author Amarger, Noelle
Alexander, M.
author_facet Amarger, Noelle
Alexander, M.
author_sort Amarger, Noelle
title Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
title_short Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
title_full Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
title_fullStr Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
title_full_unstemmed Nitrite Formation from Hydroxylamine and Oximes by Pseudomonas aeruginosa
title_sort nitrite formation from hydroxylamine and oximes by pseudomonas aeruginosa
publishDate 1968
url https://ncbi.nlm.nih.gov/pmc/articles/PMC252191/
https://ncbi.nlm.nih.gov/pubmed/4384968
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