Cellular proteins which can specifically associate with simian virus 40 small t antigen.

When crude, radiolabeled extracts of various cells were applied to homogeneous simian virus 40 small t antigen-Sepharose adsorbents, three cell proteins (57, 32, and 20 kilodaltons [kDa]) bound specifically. Each also bound to an insoluble, truncated t derivative composed of the COOH-terminal 123 re...

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Main Authors: Murphy, C I, Bikel, I, Livingston, D M
Formato: Artigo
Idioma:en
Publicado em: 1986
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC253242/
https://ncbi.nlm.nih.gov/pubmed/3016331
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spelling pubmed-2532422003-12-01 Cellular proteins which can specifically associate with simian virus 40 small t antigen. Murphy, C I Bikel, I Livingston, D M J Virol Research Article When crude, radiolabeled extracts of various cells were applied to homogeneous simian virus 40 small t antigen-Sepharose adsorbents, three cell proteins (57, 32, and 20 kilodaltons [kDa]) bound specifically. Each also bound to an insoluble, truncated t derivative composed of the COOH-terminal 123 residues of the protein. The binding of these proteins was greatly inhibited after reduction and alkylation of the t ligand. Therefore, some element of native conformation, but not all of the primary structure of t, is necessary for this binding property, which may constitute a discrete, in vitro biochemical function of this protein. Results of cell fractionation experiments suggested that the 57- and 32-kDa proteins are nonnuclear cell constituents, whereas the 20-kDa protein was closely associated with a detergent-washed nuclear fraction. Specific immunoblotting and comparative partial proteolytic digestion analyses indicated that the 57-kDa protein is tubulin, a major component of the cytoskeleton. In this regard, t and tubulin were observed to coimmunoprecipitate from crude cell extracts after incubation with monospecific anti-t antibody. Therefore, it is possible that t and tubulin interact in vivo. 1986-09 /pmc/articles/PMC253242/ /pubmed/3016331 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Murphy, C I
Bikel, I
Livingston, D M
Cellular proteins which can specifically associate with simian virus 40 small t antigen.
description When crude, radiolabeled extracts of various cells were applied to homogeneous simian virus 40 small t antigen-Sepharose adsorbents, three cell proteins (57, 32, and 20 kilodaltons [kDa]) bound specifically. Each also bound to an insoluble, truncated t derivative composed of the COOH-terminal 123 residues of the protein. The binding of these proteins was greatly inhibited after reduction and alkylation of the t ligand. Therefore, some element of native conformation, but not all of the primary structure of t, is necessary for this binding property, which may constitute a discrete, in vitro biochemical function of this protein. Results of cell fractionation experiments suggested that the 57- and 32-kDa proteins are nonnuclear cell constituents, whereas the 20-kDa protein was closely associated with a detergent-washed nuclear fraction. Specific immunoblotting and comparative partial proteolytic digestion analyses indicated that the 57-kDa protein is tubulin, a major component of the cytoskeleton. In this regard, t and tubulin were observed to coimmunoprecipitate from crude cell extracts after incubation with monospecific anti-t antibody. Therefore, it is possible that t and tubulin interact in vivo.
author Murphy, C I
Bikel, I
Livingston, D M
author_facet Murphy, C I
Bikel, I
Livingston, D M
author_sort Murphy, C I
title Cellular proteins which can specifically associate with simian virus 40 small t antigen.
title_short Cellular proteins which can specifically associate with simian virus 40 small t antigen.
title_full Cellular proteins which can specifically associate with simian virus 40 small t antigen.
title_fullStr Cellular proteins which can specifically associate with simian virus 40 small t antigen.
title_full_unstemmed Cellular proteins which can specifically associate with simian virus 40 small t antigen.
title_sort cellular proteins which can specifically associate with simian virus 40 small t antigen.
publishDate 1986
url https://ncbi.nlm.nih.gov/pmc/articles/PMC253242/
https://ncbi.nlm.nih.gov/pubmed/3016331
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