The T-antigen-binding domain of the simian virus 40 core origin of replication.

The simian virus 40 origin of replication contains a 27-base-pair palindrome with the sequence 5'-CA-GAGGC-C-GAGGC-G-GCCTC-G-GCCTC-TG-3'. The four 5'-GAGGC-3'/5'-GCCTC-3' pentanucleotides are known contact sites for simian virus 40 T-antigen binding in vitro. We used ol...

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Main Authors: Deb, S, Tsui, S, Koff, A, DeLucia, A L, Parsons, R, Tegtmeyer, P
Formato: Artigo
Idioma:en
Publicado em: 1987
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC254235/
https://ncbi.nlm.nih.gov/pubmed/3035215
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spelling pubmed-2542352003-12-08 The T-antigen-binding domain of the simian virus 40 core origin of replication. Deb, S Tsui, S Koff, A DeLucia, A L Parsons, R Tegtmeyer, P J Virol Research Article The simian virus 40 origin of replication contains a 27-base-pair palindrome with the sequence 5'-CA-GAGGC-C-GAGGC-G-GCCTC-G-GCCTC-TG-3'. The four 5'-GAGGC-3'/5'-GCCTC-3' pentanucleotides are known contact sites for simian virus 40 T-antigen binding in vitro. We used oligonucleotide-directed cassette mutagenesis to identify features of this palindrome that are important for the initiation of DNA replication in vivo. Each base pair of a pentanucleotide is crucial for DNA replication. In contrast, sequences adjacent to pentanucleotides have little or no effect on replication. Thus, the pentanucleotide is the basic functional unit, not only for T-antigen binding but also for DNA replication. All four pentanucleotides are indispensable in the initiation process. The spacing of pentanucleotides is crucial because duplication of the single base pair between binding sites has a far greater effect on replication than does substitution of the same base pair. Inversion of any pentanucleotide blocks DNA synthesis. Thus, the pentanucleotide is not a functionally symmetrical unit. We propose that each pentanucleotide positions a monomer of T antigen at the proper distance, rotation, and orientation relative to other T-antigen monomers and to other origin domains and that such positioning leads to subsequent events in replication. 1987-07 /pmc/articles/PMC254235/ /pubmed/3035215 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Deb, S
Tsui, S
Koff, A
DeLucia, A L
Parsons, R
Tegtmeyer, P
The T-antigen-binding domain of the simian virus 40 core origin of replication.
description The simian virus 40 origin of replication contains a 27-base-pair palindrome with the sequence 5'-CA-GAGGC-C-GAGGC-G-GCCTC-G-GCCTC-TG-3'. The four 5'-GAGGC-3'/5'-GCCTC-3' pentanucleotides are known contact sites for simian virus 40 T-antigen binding in vitro. We used oligonucleotide-directed cassette mutagenesis to identify features of this palindrome that are important for the initiation of DNA replication in vivo. Each base pair of a pentanucleotide is crucial for DNA replication. In contrast, sequences adjacent to pentanucleotides have little or no effect on replication. Thus, the pentanucleotide is the basic functional unit, not only for T-antigen binding but also for DNA replication. All four pentanucleotides are indispensable in the initiation process. The spacing of pentanucleotides is crucial because duplication of the single base pair between binding sites has a far greater effect on replication than does substitution of the same base pair. Inversion of any pentanucleotide blocks DNA synthesis. Thus, the pentanucleotide is not a functionally symmetrical unit. We propose that each pentanucleotide positions a monomer of T antigen at the proper distance, rotation, and orientation relative to other T-antigen monomers and to other origin domains and that such positioning leads to subsequent events in replication.
author Deb, S
Tsui, S
Koff, A
DeLucia, A L
Parsons, R
Tegtmeyer, P
author_facet Deb, S
Tsui, S
Koff, A
DeLucia, A L
Parsons, R
Tegtmeyer, P
author_sort Deb, S
title The T-antigen-binding domain of the simian virus 40 core origin of replication.
title_short The T-antigen-binding domain of the simian virus 40 core origin of replication.
title_full The T-antigen-binding domain of the simian virus 40 core origin of replication.
title_fullStr The T-antigen-binding domain of the simian virus 40 core origin of replication.
title_full_unstemmed The T-antigen-binding domain of the simian virus 40 core origin of replication.
title_sort t-antigen-binding domain of the simian virus 40 core origin of replication.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC254235/
https://ncbi.nlm.nih.gov/pubmed/3035215
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