TY  - JOUR
T1  - Manganese Superoxide Dismutase Gene Coding Region Polymorphisms Lack Clinical Incidence in General Population
A1  - Martin, Robert C.G.
LA  - English
PB  - Mary Ann Liebert, Inc., publishers
YR  - 2008
UL  - http://buscaintegrada.pucsp.br/vufind/Record/pubmed-2576292
AB  - Two functional polymorphisms within the manganese superoxide dismutase (MnSOD) gene have been reported to lead to increased oxidative stress damage. The MnSOD 58T > C single nucleotide polymorphism (SNP) within exon 3 changes isoleucine to threonine, leading to decreased thermal stability and reduced enzymatic activity in vivo and in vitro. The MnSOD 60C > T polymorphism within exon 3 changes leucine to phenylalanine, rendering the protein sensitive to redox regulation by intracellular thiols. Thus, the goal of this study was to evaluate the 58T > C and 60C > T MnSOD polymorphisms in a large case–control study. Taqman allelic discrimination assays were developed to identify the 58T > C and 60C > T SNPs in exon 3. Two hundred and eight lung cancer cases and 141 controls were evaluated for these two SNPs, and all 349 subjects were of the wild-type homozygous genotype for both 58C and 60T in exon 3. This study suggests that although the 58T > C and 60C > T polymorphisms reduce MnSOD enzymatic activity, these polymorphisms were not identified in the present case–control study population.
KW  - Original Papers
ER  -