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Multiplexed FRET to Image Multiple Signaling Events in Live Cells
We report what to our knowledge is a novel approach for simultaneous imaging of two different Förster resonance energy transfer (FRET) sensors in the same cell with minimal spectral cross talk. Previous methods based on spectral ratiometric imaging of the two FRET sensors have been limited by the av...
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| Главные авторы: | , , , , , , , , , , |
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| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
The Biophysical Society
2008
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2576366/ https://ncbi.nlm.nih.gov/pubmed/18757561 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.139204 |
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