Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.

The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol...

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Auteurs principaux: Gamazo, C, Vitas, A I, López-Goñi, I, Díaz, R, Moriyón, I
Format: Article
Langue:en
Publié: 1993
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Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC266375/
https://ncbi.nlm.nih.gov/pubmed/8308111
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spelling pubmed-2663752003-12-16 Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures. Gamazo, C Vitas, A I López-Goñi, I Díaz, R Moriyón, I J Clin Microbiol Research Article The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, D-glucose, and L-alanine), and urea in the release of CO2 was evaluated by using the reference strain Brucella melitensis 16M. In standard NR6 vials with or without blood, inocula 5 to 10 times larger (at least 265 CFU per vial) than those usually found in the blood of patients with brucellosis were necessary to produce a positive growth value (GV) in 4 days or less, and similar results were obtained with vials supplemented with the substrates listed above. GVs were consistently lower in vials with sodium polyanetholesulfonate than in vials without this agent. Vials with no blood inoculated with 265 CFU per vial showed turbidity 1 day before GVs became positive, proving that the major limiting detection factor was the low level of release of CO2 and not an inadequate growth medium. In NR6 vials buffered to pH 6.2, GVs became positive faster and were higher than those in standard vials. NR6 vials at pH 6.2 with 0.3% sodium pyruvate yielded a positive GV in the first day of bacterial turbidity. 1993-12 /pmc/articles/PMC266375/ /pubmed/8308111 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Gamazo, C
Vitas, A I
López-Goñi, I
Díaz, R
Moriyón, I
Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
description The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, D-glucose, and L-alanine), and urea in the release of CO2 was evaluated by using the reference strain Brucella melitensis 16M. In standard NR6 vials with or without blood, inocula 5 to 10 times larger (at least 265 CFU per vial) than those usually found in the blood of patients with brucellosis were necessary to produce a positive growth value (GV) in 4 days or less, and similar results were obtained with vials supplemented with the substrates listed above. GVs were consistently lower in vials with sodium polyanetholesulfonate than in vials without this agent. Vials with no blood inoculated with 265 CFU per vial showed turbidity 1 day before GVs became positive, proving that the major limiting detection factor was the low level of release of CO2 and not an inadequate growth medium. In NR6 vials buffered to pH 6.2, GVs became positive faster and were higher than those in standard vials. NR6 vials at pH 6.2 with 0.3% sodium pyruvate yielded a positive GV in the first day of bacterial turbidity.
author Gamazo, C
Vitas, A I
López-Goñi, I
Díaz, R
Moriyón, I
author_facet Gamazo, C
Vitas, A I
López-Goñi, I
Díaz, R
Moriyón, I
author_sort Gamazo, C
title Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
title_short Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
title_full Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
title_fullStr Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
title_full_unstemmed Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures.
title_sort factors affecting detection of brucella melitensis by bactec nr730, a nonradiometric system for hemocultures.
publishDate 1993
url https://ncbi.nlm.nih.gov/pmc/articles/PMC266375/
https://ncbi.nlm.nih.gov/pubmed/8308111
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