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Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens.
Results of counterimmunoelectrophoresis (CIE) were compared with those of isolation of Clostridium difficile and assay for cytotoxicity in HeLa cells. On the basis of 471 stool specimens, CIE exhibited a sensitivity of 38% and a specificity of 88% as compared with the cytotoxin assay. The predictive...
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pubmed-272702 |
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pubmed-2727022003-12-16 Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. Wu, T C Fung, J C J Clin Microbiol Research Article Results of counterimmunoelectrophoresis (CIE) were compared with those of isolation of Clostridium difficile and assay for cytotoxicity in HeLa cells. On the basis of 471 stool specimens, CIE exhibited a sensitivity of 38% and a specificity of 88% as compared with the cytotoxin assay. The predictive value of a reactive CIE results is low (17%), whereas the predictive value of a nonreactive CIE result is significant (96%) and therefore warrants its use as a screening test. In addition, stool filtrates may nonspecifically precipitate with the C. difficile antitoxin in the CIE test. Such nonspecific reactions may be identified by simultaneous electrophoresis against nonimmune serum. 1983-04 /pmc/articles/PMC272702/ /pubmed/6853688 Text en |
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US National Library of Medicine |
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PubMed Central |
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English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English |
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Article |
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Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Bacteriology Research Article Immunology General Clinical Microbiology Bacteriology Research Article Research Article Errata Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article |
| spellingShingle |
Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Bacteriology Research Article Immunology General Clinical Microbiology Bacteriology Research Article Research Article Errata Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Wu, T C Fung, J C Gallo, D Dupuis, K W Schmidt, N J Kenny, G E Fleisher, G R Collins, M Fager, S Barrett, T J Blake, P A Brown, S L Hoffman, K Llort, J M Feeley, J C Zierdt, C H Vincent, J W Falkler, W A Craig, J A Goldstein, E J Tarenzi, L A Agyare, E O Berger, J R Granström, M Julander, I G Hedström, S A Möllby, R Staneck, J L Vincelette, J Lamothe, F Polk, E A Kenny, G E Dunsmoor, C L Stamm, W E Tam, M Koester, M Cles, L Sechter, I Shmilovitz, M Altmann, G Seligmann, R Kretzer, B Braunstein, I Gerichter, C B Gilbreath, M J Pavanand, K MacDermott, R P Ussery, M Burke, D S Nimmannitya, S Tulyayon, S Nilheden, E Jeansson, S Vahlne, A Cheasty, T Rowe, B Fayram, S L Nakasone, A Aarnaes, S Zartarian, M Peterson, E M de la Maza, L M Lecce, J G Clare, D A Balsbaugh, R K Collier, D N Reynolds, Judith G. Silva, Ellen McCormack, William M. Dube, S D Rautelin, Hilpi Kosunen, Timo U. Kuehne, Ralph W. Mittal, A. Sathish, M. Seshadri, P. S. Nath, Indira Mishra, S K Falkenberg, S Masihi, K N Zabransky, R J Randall, E Sutter, V L Birk, R J Westenfelder, G Emmerman, J Ghoneim, A T Jamjoom, G A Ashton, F E Ryan, A Diena, B Jennings, H J Stephens, L R Humphrey, J D Little, P B Barnum, D A Skurrie, I J Gilbert, G L Lévy-Frébault, V Daffé, M Goh, K S Lanéelle, M A Asselineau, C David, H L Grundy, M S Cartwright-Taylor, L Lundin, L Thors, C Huldt, G Wikström, M B Dahlén, G Linde, A Turunen, H J Polonelli, L Archibusacci, C Sestito, M Morace, G Imaizumi, A Suzuki, Y Ono, S Sato, H Sato, Y Mittal, K R Higgins, R Lariviere, S James, R Serafim, M B Monteiro, A R de Castro, A F Chang, G T Ellner, P D Woolfrey, B F Lally, R T Quall, C O Boteler, W L Luipersbeck, P M Fuccillo, D A O'Beirne, A J Widen, R Klein, T Lee, I Friedman, H Buesching, W J Svirbely, J R Ayers, L W Cooperstock, M Riegle, L Woodruff, C W Onderdonk, A Lemon, S M Binn, L N Marchwicki, R H Mathews, H M Healy, G R Lynn, M Katz, M A Santucci, T F Finn, M P Ohlin, A Schachter, J Martin, W T Patton, C M Morris, G K Potter, M E Puhr, N D Lamberg, R E Schell, R F LeFrock, J L Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| description |
Results of counterimmunoelectrophoresis (CIE) were compared with those of isolation of Clostridium difficile and assay for cytotoxicity in HeLa cells. On the basis of 471 stool specimens, CIE exhibited a sensitivity of 38% and a specificity of 88% as compared with the cytotoxin assay. The predictive value of a reactive CIE results is low (17%), whereas the predictive value of a nonreactive CIE result is significant (96%) and therefore warrants its use as a screening test. In addition, stool filtrates may nonspecifically precipitate with the C. difficile antitoxin in the CIE test. Such nonspecific reactions may be identified by simultaneous electrophoresis against nonimmune serum. |
| author |
Wu, T C Fung, J C Gallo, D Dupuis, K W Schmidt, N J Kenny, G E Fleisher, G R Collins, M Fager, S Barrett, T J Blake, P A Brown, S L Hoffman, K Llort, J M Feeley, J C Zierdt, C H Vincent, J W Falkler, W A Craig, J A Goldstein, E J Tarenzi, L A Agyare, E O Berger, J R Granström, M Julander, I G Hedström, S A Möllby, R Staneck, J L Vincelette, J Lamothe, F Polk, E A Kenny, G E Dunsmoor, C L Stamm, W E Tam, M Koester, M Cles, L Sechter, I Shmilovitz, M Altmann, G Seligmann, R Kretzer, B Braunstein, I Gerichter, C B Gilbreath, M J Pavanand, K MacDermott, R P Ussery, M Burke, D S Nimmannitya, S Tulyayon, S Nilheden, E Jeansson, S Vahlne, A Cheasty, T Rowe, B Fayram, S L Nakasone, A Aarnaes, S Zartarian, M Peterson, E M de la Maza, L M Lecce, J G Clare, D A Balsbaugh, R K Collier, D N Reynolds, Judith G. Silva, Ellen McCormack, William M. Dube, S D Rautelin, Hilpi Kosunen, Timo U. Kuehne, Ralph W. Mittal, A. Sathish, M. Seshadri, P. S. Nath, Indira Mishra, S K Falkenberg, S Masihi, K N Zabransky, R J Randall, E Sutter, V L Birk, R J Westenfelder, G Emmerman, J Ghoneim, A T Jamjoom, G A Ashton, F E Ryan, A Diena, B Jennings, H J Stephens, L R Humphrey, J D Little, P B Barnum, D A Skurrie, I J Gilbert, G L Lévy-Frébault, V Daffé, M Goh, K S Lanéelle, M A Asselineau, C David, H L Grundy, M S Cartwright-Taylor, L Lundin, L Thors, C Huldt, G Wikström, M B Dahlén, G Linde, A Turunen, H J Polonelli, L Archibusacci, C Sestito, M Morace, G Imaizumi, A Suzuki, Y Ono, S Sato, H Sato, Y Mittal, K R Higgins, R Lariviere, S James, R Serafim, M B Monteiro, A R de Castro, A F Chang, G T Ellner, P D Woolfrey, B F Lally, R T Quall, C O Boteler, W L Luipersbeck, P M Fuccillo, D A O'Beirne, A J Widen, R Klein, T Lee, I Friedman, H Buesching, W J Svirbely, J R Ayers, L W Cooperstock, M Riegle, L Woodruff, C W Onderdonk, A Lemon, S M Binn, L N Marchwicki, R H Mathews, H M Healy, G R Lynn, M Katz, M A Santucci, T F Finn, M P Ohlin, A Schachter, J Martin, W T Patton, C M Morris, G K Potter, M E Puhr, N D Lamberg, R E Schell, R F LeFrock, J L |
| author_facet |
Wu, T C Fung, J C Gallo, D Dupuis, K W Schmidt, N J Kenny, G E Fleisher, G R Collins, M Fager, S Barrett, T J Blake, P A Brown, S L Hoffman, K Llort, J M Feeley, J C Zierdt, C H Vincent, J W Falkler, W A Craig, J A Goldstein, E J Tarenzi, L A Agyare, E O Berger, J R Granström, M Julander, I G Hedström, S A Möllby, R Staneck, J L Vincelette, J Lamothe, F Polk, E A Kenny, G E Dunsmoor, C L Stamm, W E Tam, M Koester, M Cles, L Sechter, I Shmilovitz, M Altmann, G Seligmann, R Kretzer, B Braunstein, I Gerichter, C B Gilbreath, M J Pavanand, K MacDermott, R P Ussery, M Burke, D S Nimmannitya, S Tulyayon, S Nilheden, E Jeansson, S Vahlne, A Cheasty, T Rowe, B Fayram, S L Nakasone, A Aarnaes, S Zartarian, M Peterson, E M de la Maza, L M Lecce, J G Clare, D A Balsbaugh, R K Collier, D N Reynolds, Judith G. Silva, Ellen McCormack, William M. Dube, S D Rautelin, Hilpi Kosunen, Timo U. Kuehne, Ralph W. Mittal, A. Sathish, M. Seshadri, P. S. Nath, Indira Mishra, S K Falkenberg, S Masihi, K N Zabransky, R J Randall, E Sutter, V L Birk, R J Westenfelder, G Emmerman, J Ghoneim, A T Jamjoom, G A Ashton, F E Ryan, A Diena, B Jennings, H J Stephens, L R Humphrey, J D Little, P B Barnum, D A Skurrie, I J Gilbert, G L Lévy-Frébault, V Daffé, M Goh, K S Lanéelle, M A Asselineau, C David, H L Grundy, M S Cartwright-Taylor, L Lundin, L Thors, C Huldt, G Wikström, M B Dahlén, G Linde, A Turunen, H J Polonelli, L Archibusacci, C Sestito, M Morace, G Imaizumi, A Suzuki, Y Ono, S Sato, H Sato, Y Mittal, K R Higgins, R Lariviere, S James, R Serafim, M B Monteiro, A R de Castro, A F Chang, G T Ellner, P D Woolfrey, B F Lally, R T Quall, C O Boteler, W L Luipersbeck, P M Fuccillo, D A O'Beirne, A J Widen, R Klein, T Lee, I Friedman, H Buesching, W J Svirbely, J R Ayers, L W Cooperstock, M Riegle, L Woodruff, C W Onderdonk, A Lemon, S M Binn, L N Marchwicki, R H Mathews, H M Healy, G R Lynn, M Katz, M A Santucci, T F Finn, M P Ohlin, A Schachter, J Martin, W T Patton, C M Morris, G K Potter, M E Puhr, N D Lamberg, R E Schell, R F LeFrock, J L |
| author_sort |
Wu, T C |
| title |
Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| title_short |
Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| title_full |
Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| title_fullStr |
Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| title_full_unstemmed |
Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens. |
| title_sort |
evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of clostridium difficile-associated colitis in clinical specimens. |
| publishDate |
1983 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC272702/ https://ncbi.nlm.nih.gov/pubmed/6853688 https://ncbi.nlm.nih.gov/pmc/articles/PMC272703/ https://ncbi.nlm.nih.gov/pubmed/6406539 https://ncbi.nlm.nih.gov/pmc/articles/PMC272704/ https://ncbi.nlm.nih.gov/pubmed/6304142 https://ncbi.nlm.nih.gov/pmc/articles/PMC272705/ https://ncbi.nlm.nih.gov/pubmed/6853689 https://ncbi.nlm.nih.gov/pmc/articles/PMC272706/ https://ncbi.nlm.nih.gov/pubmed/6853690 https://ncbi.nlm.nih.gov/pmc/articles/PMC272707/ https://ncbi.nlm.nih.gov/pubmed/6406540 https://ncbi.nlm.nih.gov/pmc/articles/PMC272708/ https://ncbi.nlm.nih.gov/pubmed/6343421 https://ncbi.nlm.nih.gov/pmc/articles/PMC272709/ https://ncbi.nlm.nih.gov/pubmed/6853691 https://ncbi.nlm.nih.gov/pmc/articles/PMC272710/ https://ncbi.nlm.nih.gov/pubmed/6343422 https://ncbi.nlm.nih.gov/pmc/articles/PMC272711/ https://ncbi.nlm.nih.gov/pubmed/6343423 https://ncbi.nlm.nih.gov/pmc/articles/PMC272712/ https://ncbi.nlm.nih.gov/pubmed/6189852 https://ncbi.nlm.nih.gov/pmc/articles/PMC272713/ https://ncbi.nlm.nih.gov/pubmed/6853692 https://ncbi.nlm.nih.gov/pmc/articles/PMC272714/ https://ncbi.nlm.nih.gov/pubmed/6602147 https://ncbi.nlm.nih.gov/pmc/articles/PMC272715/ https://ncbi.nlm.nih.gov/pubmed/6189853 https://ncbi.nlm.nih.gov/pmc/articles/PMC272716/ https://ncbi.nlm.nih.gov/pubmed/6189854 https://ncbi.nlm.nih.gov/pmc/articles/PMC272717/ https://ncbi.nlm.nih.gov/pubmed/6343424 https://ncbi.nlm.nih.gov/pmc/articles/PMC272718/ https://ncbi.nlm.nih.gov/pubmed/6343425 https://ncbi.nlm.nih.gov/pmc/articles/PMC272719/ https://ncbi.nlm.nih.gov/pubmed/6853693 https://ncbi.nlm.nih.gov/pmc/articles/PMC272720/ https://ncbi.nlm.nih.gov/pubmed/6853694 https://ncbi.nlm.nih.gov/pmc/articles/PMC272721/ https://ncbi.nlm.nih.gov/pubmed/6343426 https://ncbi.nlm.nih.gov/pmc/articles/PMC272722/ https://ncbi.nlm.nih.gov/pubmed/6343427 https://ncbi.nlm.nih.gov/pmc/articles/PMC272723/ https://ncbi.nlm.nih.gov/pubmed/6343428 https://ncbi.nlm.nih.gov/pmc/articles/PMC272724/ https://ncbi.nlm.nih.gov/pubmed/6406541 https://ncbi.nlm.nih.gov/pmc/articles/PMC272725/ https://ncbi.nlm.nih.gov/pubmed/6222065 https://ncbi.nlm.nih.gov/pmc/articles/PMC272726/ https://ncbi.nlm.nih.gov/pmc/articles/PMC272731/ https://ncbi.nlm.nih.gov/pubmed/6863496 https://ncbi.nlm.nih.gov/pmc/articles/PMC272732/ https://ncbi.nlm.nih.gov/pubmed/6408117 https://ncbi.nlm.nih.gov/pmc/articles/PMC272733/ https://ncbi.nlm.nih.gov/pubmed/6408118 https://ncbi.nlm.nih.gov/pmc/articles/PMC272734/ https://ncbi.nlm.nih.gov/pubmed/6863497 https://ncbi.nlm.nih.gov/pmc/articles/PMC272735/ https://ncbi.nlm.nih.gov/pubmed/6190837 https://ncbi.nlm.nih.gov/pmc/articles/PMC272736/ https://ncbi.nlm.nih.gov/pubmed/6306046 https://ncbi.nlm.nih.gov/pmc/articles/PMC272737/ https://ncbi.nlm.nih.gov/pubmed/6345573 https://ncbi.nlm.nih.gov/pmc/articles/PMC272738/ https://ncbi.nlm.nih.gov/pubmed/6345574 https://ncbi.nlm.nih.gov/pmc/articles/PMC272739/ https://ncbi.nlm.nih.gov/pubmed/6345575 https://ncbi.nlm.nih.gov/pmc/articles/PMC272740/ https://ncbi.nlm.nih.gov/pubmed/6306047 https://ncbi.nlm.nih.gov/pmc/articles/PMC272741/ https://ncbi.nlm.nih.gov/pubmed/6190838 https://ncbi.nlm.nih.gov/pmc/articles/PMC272742/ https://ncbi.nlm.nih.gov/pubmed/6345576 https://ncbi.nlm.nih.gov/pmc/articles/PMC272743/ https://ncbi.nlm.nih.gov/pubmed/6863498 https://ncbi.nlm.nih.gov/pmc/articles/PMC272744/ https://ncbi.nlm.nih.gov/pubmed/6863499 https://ncbi.nlm.nih.gov/pmc/articles/PMC272745/ https://ncbi.nlm.nih.gov/pubmed/6345577 https://ncbi.nlm.nih.gov/pmc/articles/PMC272746/ https://ncbi.nlm.nih.gov/pubmed/6863500 https://ncbi.nlm.nih.gov/pmc/articles/PMC272747/ https://ncbi.nlm.nih.gov/pubmed/6863501 https://ncbi.nlm.nih.gov/pmc/articles/PMC272748/ https://ncbi.nlm.nih.gov/pubmed/6345578 https://ncbi.nlm.nih.gov/pmc/articles/PMC272749/ https://ncbi.nlm.nih.gov/pubmed/6863502 https://ncbi.nlm.nih.gov/pmc/articles/PMC272750/ https://ncbi.nlm.nih.gov/pubmed/6306048 https://ncbi.nlm.nih.gov/pmc/articles/PMC272751/ https://ncbi.nlm.nih.gov/pubmed/6408119 https://ncbi.nlm.nih.gov/pmc/articles/PMC272752/ https://ncbi.nlm.nih.gov/pubmed/6602808 https://ncbi.nlm.nih.gov/pmc/articles/PMC272753/ https://ncbi.nlm.nih.gov/pubmed/6863503 https://ncbi.nlm.nih.gov/pmc/articles/PMC272754/ https://ncbi.nlm.nih.gov/pubmed/6863504 https://ncbi.nlm.nih.gov/pmc/articles/PMC272755/ https://ncbi.nlm.nih.gov/pubmed/6863505 |
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