Differential expression of complement C3 and C4 in the human kidney.

Complement activation is associated with a variety of immunologically-mediated renal diseases. Proximal tubular epithelial cells in situ constitutively express messenger RNA for C4 of the complement system. These same epithelial cells in culture have been reported to contain message for C3 and to se...

Mô tả đầy đủ

Đã lưu trong:
Chi tiết về thư mục
Những tác giả chính: Welch, T R, Beischel, L S, Witte, D P
Định dạng: Bài viết
Ngôn ngữ:en
Được phát hành: 1993
Những chủ đề:
Research Article
Truy cập trực tuyến:https://ncbi.nlm.nih.gov/pmc/articles/PMC288290/
https://ncbi.nlm.nih.gov/pubmed/8376597
Các nhãn: Thêm thẻ
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
id pubmed-288290
record_format dspace
spelling pubmed-2882902004-07-07 Differential expression of complement C3 and C4 in the human kidney. Welch, T R Beischel, L S Witte, D P J Clin Invest Research Article Complement activation is associated with a variety of immunologically-mediated renal diseases. Proximal tubular epithelial cells in situ constitutively express messenger RNA for C4 of the complement system. These same epithelial cells in culture have been reported to contain message for C3 and to secrete this protein when stimulated by IL-2. The present study compared the in situ localization of C3 and C4 message in parallel in a variety of renal biopsy and nephrectomy specimens. All adequate tissue samples (n = 23) had C4 mRNA throughout in the cortical tubular epithelium. Although C3 message was also expressed in tubular epithelial cells, there was much greater variation in its distribution. mRNA for C3 was not detected in histologically normal specimens (n = 4) either by in situ or Northern hybridization. Focal C3 message correlated with focal histologic abnormalities (e.g., focal glomerulosclerosis), while more generalized C3 signal occurred in specimens with more diffuse inflammatory processes (e.g., SLE). Infiltrating inflammatory cells and cells of the glomeruli were uniformly negative for C3 (and C4) message. Tubular C3 and C4 mRNA appeared to be translated, since selected specimens showed cytoplasmic staining by monoclonal antibodies to C3c and C4c. These observations are consistent with the hypothesis that local production of inflammatory mediators could induce C3 synthesis in the renal interstitium, with the possibility that subsequent complement activation could enhance the pathogenic process. 1993-09 /pmc/articles/PMC288290/ /pubmed/8376597 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Welch, T R
Beischel, L S
Witte, D P
Differential expression of complement C3 and C4 in the human kidney.
description Complement activation is associated with a variety of immunologically-mediated renal diseases. Proximal tubular epithelial cells in situ constitutively express messenger RNA for C4 of the complement system. These same epithelial cells in culture have been reported to contain message for C3 and to secrete this protein when stimulated by IL-2. The present study compared the in situ localization of C3 and C4 message in parallel in a variety of renal biopsy and nephrectomy specimens. All adequate tissue samples (n = 23) had C4 mRNA throughout in the cortical tubular epithelium. Although C3 message was also expressed in tubular epithelial cells, there was much greater variation in its distribution. mRNA for C3 was not detected in histologically normal specimens (n = 4) either by in situ or Northern hybridization. Focal C3 message correlated with focal histologic abnormalities (e.g., focal glomerulosclerosis), while more generalized C3 signal occurred in specimens with more diffuse inflammatory processes (e.g., SLE). Infiltrating inflammatory cells and cells of the glomeruli were uniformly negative for C3 (and C4) message. Tubular C3 and C4 mRNA appeared to be translated, since selected specimens showed cytoplasmic staining by monoclonal antibodies to C3c and C4c. These observations are consistent with the hypothesis that local production of inflammatory mediators could induce C3 synthesis in the renal interstitium, with the possibility that subsequent complement activation could enhance the pathogenic process.
author Welch, T R
Beischel, L S
Witte, D P
author_facet Welch, T R
Beischel, L S
Witte, D P
author_sort Welch, T R
title Differential expression of complement C3 and C4 in the human kidney.
title_short Differential expression of complement C3 and C4 in the human kidney.
title_full Differential expression of complement C3 and C4 in the human kidney.
title_fullStr Differential expression of complement C3 and C4 in the human kidney.
title_full_unstemmed Differential expression of complement C3 and C4 in the human kidney.
title_sort differential expression of complement c3 and c4 in the human kidney.
publishDate 1993
url https://ncbi.nlm.nih.gov/pmc/articles/PMC288290/
https://ncbi.nlm.nih.gov/pubmed/8376597
_version_ 1759373571433431040

Những quyển sách tương tự