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In vivo labelling of functional ribosomes reveals spatial regulation during starvation in Podospora anserina
BACKGROUND: To date, in eukaryotes, ribosomal protein expression is known to be regulated at the transcriptional and/or translational levels. But other forms of regulation may be possible. RESULTS: Here, we report the successful tagging of functional ribosomal particles with a S7-GFP chimaeric prote...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
BioMed Central
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC29079/ https://ncbi.nlm.nih.gov/pubmed/11112985 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2156-1-3 |
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| Resumo: | BACKGROUND: To date, in eukaryotes, ribosomal protein expression is known to be regulated at the transcriptional and/or translational levels. But other forms of regulation may be possible. RESULTS: Here, we report the successful tagging of functional ribosomal particles with a S7-GFP chimaeric protein, making it possible to observe in vivo ribosome dynamics in the filamentous fungus Podospora anserina. Microscopic observations revealed a novel kind of ribosomal protein regulation during the passage between cell growth and stationary phases, with a transient accumulation of ribosomal proteins and/or ribosome subunits in the nucleus, possibly the nucleolus, being observed at the beginning of stationary phase. CONCLUSION: Nuclear sequestration can be another level of ribosomal protein regulation in eukaryotic cells.This may contribute to the regulation of cell growth and division. |
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