Luminal vasopressin modulates transport in the rabbit cortical collecting duct.

We explored the action of luminal AVP in rabbit CCD perfused in vitro at 37 degrees C. Nanomolar concentrations of luminal AVP induced a sustained hyperpolarization of transepithelial voltage (Vt) in contrast to a transient hyperpolarization caused by basolateral AVP. 10 microM basolateral ouabain a...

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Prif Awduron: Ando, Y, Tabei, K, Asano, Y
Fformat: Erthygl
Iaith:en
Cyhoeddwyd: 1991
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Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC295494/
https://ncbi.nlm.nih.gov/pubmed/1885780
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spelling pubmed-2954942004-04-09 Luminal vasopressin modulates transport in the rabbit cortical collecting duct. Ando, Y Tabei, K Asano, Y J Clin Invest Research Article We explored the action of luminal AVP in rabbit CCD perfused in vitro at 37 degrees C. Nanomolar concentrations of luminal AVP induced a sustained hyperpolarization of transepithelial voltage (Vt) in contrast to a transient hyperpolarization caused by basolateral AVP. 10 microM basolateral ouabain abolished the latter but not the former change in Vt. Despite a sustained hyperpolarization (from -20.7 +/- 2.9 to -34.1 +/- 4.7 mV; P less than 0.01), 10 nM luminal AVP only slightly altered net Na+ and K+ fluxes (7.6% stimulation and no significant change, respectively). Instead, luminal AVP appeared to modulate an acetazolamide-sensitive electrogenic ion transport because 200 microM basolateral acetazolamide suppressed the luminal AVP-induced hyperpolarization (percentage of Vt from -50.4 +/- 10.8 to -5.1 +/- 1.4; P less than 0.005). In terms of water transport, 10 nM luminal AVP did not change hydraulic conductivity (Lp, x 10(-7) cm/atm per s) (from 3.9 +/- 0.8 to 5.0 +/- 1.2), but suppressed the increase in Lp induced by 20 pM basolateral AVP (134.9 +/- 19.2 vs. 204.3 +/- 21.1 in control; P less than 0.05). These findings demonstrate distinct luminal action of AVP, suggesting amphilateral regulation of epithelial transport by AVP in the CCD. 1991-09 /pmc/articles/PMC295494/ /pubmed/1885780 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Ando, Y
Tabei, K
Asano, Y
Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
description We explored the action of luminal AVP in rabbit CCD perfused in vitro at 37 degrees C. Nanomolar concentrations of luminal AVP induced a sustained hyperpolarization of transepithelial voltage (Vt) in contrast to a transient hyperpolarization caused by basolateral AVP. 10 microM basolateral ouabain abolished the latter but not the former change in Vt. Despite a sustained hyperpolarization (from -20.7 +/- 2.9 to -34.1 +/- 4.7 mV; P less than 0.01), 10 nM luminal AVP only slightly altered net Na+ and K+ fluxes (7.6% stimulation and no significant change, respectively). Instead, luminal AVP appeared to modulate an acetazolamide-sensitive electrogenic ion transport because 200 microM basolateral acetazolamide suppressed the luminal AVP-induced hyperpolarization (percentage of Vt from -50.4 +/- 10.8 to -5.1 +/- 1.4; P less than 0.005). In terms of water transport, 10 nM luminal AVP did not change hydraulic conductivity (Lp, x 10(-7) cm/atm per s) (from 3.9 +/- 0.8 to 5.0 +/- 1.2), but suppressed the increase in Lp induced by 20 pM basolateral AVP (134.9 +/- 19.2 vs. 204.3 +/- 21.1 in control; P less than 0.05). These findings demonstrate distinct luminal action of AVP, suggesting amphilateral regulation of epithelial transport by AVP in the CCD.
author Ando, Y
Tabei, K
Asano, Y
author_facet Ando, Y
Tabei, K
Asano, Y
author_sort Ando, Y
title Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
title_short Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
title_full Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
title_fullStr Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
title_full_unstemmed Luminal vasopressin modulates transport in the rabbit cortical collecting duct.
title_sort luminal vasopressin modulates transport in the rabbit cortical collecting duct.
publishDate 1991
url https://ncbi.nlm.nih.gov/pmc/articles/PMC295494/
https://ncbi.nlm.nih.gov/pubmed/1885780
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