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c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.

Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The co...

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Main Authors: Iguchi-Ariga, S M, Itani, T, Yamaguchi, M, Ariga, H, de Wind, N, Parren, P, Stuitje, A R, Meijer, M, Bower, M, Summers, M F, Powell, C, Shinozuka, K, Regan, J B, Zon, G, Wilson, W D, Geisen, R M, Fatscher, H P, Fuchs, E, Hinrichs, W, Steifa, M, Saenger, W, Eckstein, F, Yeung, A T, Jones, B K, Capraro, M, Chu, T, Tung, C S, Harvey, S C, Hong, H J, Yoo, S H, Yoo, O J, Imai, R, Sekiguchi, T, Nosoh, Y, Tsuda, K, Seidel, U, Bober, E, Winter, B, Lenz, S, Lohse, P, Arnold, H H, Lalanne, J L, Lucero, M, le Moullec, J M, Smith, W J, Tunnacliffe, A, Rabbitts, T H, Shimada, T, McAlister-Henn, L, Blaber, M, Bradshaw, R A, Nisco, S J, Janson, L, Bark, C, Pettersson, U, Johnston, L H, White, J H, Johnson, A L, Lucchini, G, Plevani, P, Van Dyke, M W, Roeder, R G, Rostas, K, Morton, S J, Picksley, S M, Lloyd, R G, Lee, K L, Pentecost, B T, D'Anna, J A, Tobey, R A, Gurley, L R, Dixon, G H, Po, T, Steger, G, Rosenbaum, V, Kaper, J, Riesner, D, Esposito, F, Sinden, R R, Larsen, J E, Albrechtsen, B, Valentin-Hansen, P, Makaroff, C A, Palmer, J D, Sands, J F, Cummings, H S, Sacerdot, C, Dondon, L, Grunberg-Manago, M, Hershey, J W, Poncz, M, Sutton, M, Delgrosso, K, Schwartz, E, Surrey, S, Dworniczak, B, Mirault, M E, Paulson, K E, Matera, A G, Deka, N, Schmid, C W, Berends, T, Gamble, P E, Mullet, J E, Shimamoto, N, Ikushima, N, Utiyama, H, Tachibana, H, Horie, K, Leavitt, M C, Ito, J, Zhou, K, Brisco, P R, Hinkkanen, A E, Kohlhaw, G B, Li, P, Medon, P P, Skingle, D C, Lanser, J A, Symons, R H, Hoffmann, P U, McLaughlin, L W, Zuckermann, R, Corey, D, Schultz, P, Levinson, G, Gutman, G A, Klig, L S, Crawford, I P, Yanofsky, C, Ghosh, S S, Musso, G F, Gingeras, T R, Kwoh, D Y, Davis, G R, Koller, H T, Frondorf, K A, Maschner, P D, Vaughn, J C, Chapman, K A, Burgess, R R, Poljak, L G, Gralla, J D, Saez, L J, Gianola, K M, McNally, E M, Feghali, R, Eddy, R, Shows, T B, Leinwand, L A, Andrews, G K, Adamson, E D, Stange, N, Gross, H J, Beier, H, Wilhelm, M L, Wilhelm, F X, Hall, R M, Ksenzenko, V N, Shlyapnikov, M G, Azbarov, V G, Garcia, O, Kryukov, V M, Bayev, A A, Potter, S S, Lloyd, J A, Seto, N O, Hayashi, S, Tener, G M
Formato: Artigo
Idioma:English
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Publicado em: 1987
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305925/
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spelling pubmed-3059252004-02-25 c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. Iguchi-Ariga, S M Itani, T Yamaguchi, M Ariga, H Nucleic Acids Res Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. 1987-06-25 /pmc/articles/PMC305925/ /pubmed/3037484 Text en
institution US National Library of Medicine
collection PubMed Central
language English
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description Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
author Iguchi-Ariga, S M
Itani, T
Yamaguchi, M
Ariga, H
de Wind, N
Parren, P
Stuitje, A R
Meijer, M
Bower, M
Summers, M F
Powell, C
Shinozuka, K
Regan, J B
Zon, G
Wilson, W D
Geisen, R M
Fatscher, H P
Fuchs, E
Hinrichs, W
Steifa, M
Saenger, W
Eckstein, F
Yeung, A T
Jones, B K
Capraro, M
Chu, T
Tung, C S
Harvey, S C
Hong, H J
Yoo, S H
Yoo, O J
Imai, R
Sekiguchi, T
Nosoh, Y
Tsuda, K
Seidel, U
Bober, E
Winter, B
Lenz, S
Lohse, P
Arnold, H H
Lalanne, J L
Lucero, M
le Moullec, J M
Smith, W J
Tunnacliffe, A
Rabbitts, T H
Shimada, T
McAlister-Henn, L
Blaber, M
Bradshaw, R A
Nisco, S J
Janson, L
Bark, C
Pettersson, U
Johnston, L H
White, J H
Johnson, A L
Lucchini, G
Plevani, P
Van Dyke, M W
Roeder, R G
Rostas, K
Morton, S J
Picksley, S M
Lloyd, R G
Lee, K L
Pentecost, B T
D'Anna, J A
Tobey, R A
Gurley, L R
Dixon, G H
Po, T
Steger, G
Rosenbaum, V
Kaper, J
Riesner, D
Steger, G
Po, T
Kaper, J
Riesner, D
Esposito, F
Sinden, R R
Larsen, J E
Albrechtsen, B
Valentin-Hansen, P
Makaroff, C A
Palmer, J D
Sands, J F
Cummings, H S
Sacerdot, C
Dondon, L
Grunberg-Manago, M
Hershey, J W
Poncz, M
Sutton, M
Delgrosso, K
Schwartz, E
Surrey, S
Dworniczak, B
Mirault, M E
Paulson, K E
Matera, A G
Deka, N
Schmid, C W
Berends, T
Gamble, P E
Mullet, J E
Shimamoto, N
Ikushima, N
Utiyama, H
Tachibana, H
Horie, K
Leavitt, M C
Ito, J
Zhou, K
Brisco, P R
Hinkkanen, A E
Kohlhaw, G B
Li, P
Medon, P P
Skingle, D C
Lanser, J A
Symons, R H
Hoffmann, P U
McLaughlin, L W
Zuckermann, R
Corey, D
Schultz, P
Levinson, G
Gutman, G A
Klig, L S
Crawford, I P
Yanofsky, C
Ghosh, S S
Musso, G F
Gingeras, T R
Kwoh, D Y
Davis, G R
Koller, H T
Frondorf, K A
Maschner, P D
Vaughn, J C
Chapman, K A
Burgess, R R
Poljak, L G
Gralla, J D
Saez, L J
Gianola, K M
McNally, E M
Feghali, R
Eddy, R
Shows, T B
Leinwand, L A
Andrews, G K
Adamson, E D
Stange, N
Gross, H J
Beier, H
Wilhelm, M L
Wilhelm, F X
Hall, R M
Ksenzenko, V N
Shlyapnikov, M G
Azbarov, V G
Garcia, O
Kryukov, V M
Bayev, A A
Potter, S S
Lloyd, J A
Seto, N O
Hayashi, S
Tener, G M
spellingShingle Iguchi-Ariga, S M
Itani, T
Yamaguchi, M
Ariga, H
de Wind, N
Parren, P
Stuitje, A R
Meijer, M
Bower, M
Summers, M F
Powell, C
Shinozuka, K
Regan, J B
Zon, G
Wilson, W D
Geisen, R M
Fatscher, H P
Fuchs, E
Hinrichs, W
Steifa, M
Saenger, W
Eckstein, F
Yeung, A T
Jones, B K
Capraro, M
Chu, T
Tung, C S
Harvey, S C
Hong, H J
Yoo, S H
Yoo, O J
Imai, R
Sekiguchi, T
Nosoh, Y
Tsuda, K
Seidel, U
Bober, E
Winter, B
Lenz, S
Lohse, P
Arnold, H H
Lalanne, J L
Lucero, M
le Moullec, J M
Smith, W J
Tunnacliffe, A
Rabbitts, T H
Shimada, T
McAlister-Henn, L
Blaber, M
Bradshaw, R A
Nisco, S J
Janson, L
Bark, C
Pettersson, U
Johnston, L H
White, J H
Johnson, A L
Lucchini, G
Plevani, P
Van Dyke, M W
Roeder, R G
Rostas, K
Morton, S J
Picksley, S M
Lloyd, R G
Lee, K L
Pentecost, B T
D'Anna, J A
Tobey, R A
Gurley, L R
Dixon, G H
Po, T
Steger, G
Rosenbaum, V
Kaper, J
Riesner, D
Steger, G
Po, T
Kaper, J
Riesner, D
Esposito, F
Sinden, R R
Larsen, J E
Albrechtsen, B
Valentin-Hansen, P
Makaroff, C A
Palmer, J D
Sands, J F
Cummings, H S
Sacerdot, C
Dondon, L
Grunberg-Manago, M
Hershey, J W
Poncz, M
Sutton, M
Delgrosso, K
Schwartz, E
Surrey, S
Dworniczak, B
Mirault, M E
Paulson, K E
Matera, A G
Deka, N
Schmid, C W
Berends, T
Gamble, P E
Mullet, J E
Shimamoto, N
Ikushima, N
Utiyama, H
Tachibana, H
Horie, K
Leavitt, M C
Ito, J
Zhou, K
Brisco, P R
Hinkkanen, A E
Kohlhaw, G B
Li, P
Medon, P P
Skingle, D C
Lanser, J A
Symons, R H
Hoffmann, P U
McLaughlin, L W
Zuckermann, R
Corey, D
Schultz, P
Levinson, G
Gutman, G A
Klig, L S
Crawford, I P
Yanofsky, C
Ghosh, S S
Musso, G F
Gingeras, T R
Kwoh, D Y
Davis, G R
Koller, H T
Frondorf, K A
Maschner, P D
Vaughn, J C
Chapman, K A
Burgess, R R
Poljak, L G
Gralla, J D
Saez, L J
Gianola, K M
McNally, E M
Feghali, R
Eddy, R
Shows, T B
Leinwand, L A
Andrews, G K
Adamson, E D
Stange, N
Gross, H J
Beier, H
Wilhelm, M L
Wilhelm, F X
Hall, R M
Ksenzenko, V N
Shlyapnikov, M G
Azbarov, V G
Garcia, O
Kryukov, V M
Bayev, A A
Potter, S S
Lloyd, J A
Seto, N O
Hayashi, S
Tener, G M
c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
author_facet Iguchi-Ariga, S M
Itani, T
Yamaguchi, M
Ariga, H
de Wind, N
Parren, P
Stuitje, A R
Meijer, M
Bower, M
Summers, M F
Powell, C
Shinozuka, K
Regan, J B
Zon, G
Wilson, W D
Geisen, R M
Fatscher, H P
Fuchs, E
Hinrichs, W
Steifa, M
Saenger, W
Eckstein, F
Yeung, A T
Jones, B K
Capraro, M
Chu, T
Tung, C S
Harvey, S C
Hong, H J
Yoo, S H
Yoo, O J
Imai, R
Sekiguchi, T
Nosoh, Y
Tsuda, K
Seidel, U
Bober, E
Winter, B
Lenz, S
Lohse, P
Arnold, H H
Lalanne, J L
Lucero, M
le Moullec, J M
Smith, W J
Tunnacliffe, A
Rabbitts, T H
Shimada, T
McAlister-Henn, L
Blaber, M
Bradshaw, R A
Nisco, S J
Janson, L
Bark, C
Pettersson, U
Johnston, L H
White, J H
Johnson, A L
Lucchini, G
Plevani, P
Van Dyke, M W
Roeder, R G
Rostas, K
Morton, S J
Picksley, S M
Lloyd, R G
Lee, K L
Pentecost, B T
D'Anna, J A
Tobey, R A
Gurley, L R
Dixon, G H
Po, T
Steger, G
Rosenbaum, V
Kaper, J
Riesner, D
Steger, G
Po, T
Kaper, J
Riesner, D
Esposito, F
Sinden, R R
Larsen, J E
Albrechtsen, B
Valentin-Hansen, P
Makaroff, C A
Palmer, J D
Sands, J F
Cummings, H S
Sacerdot, C
Dondon, L
Grunberg-Manago, M
Hershey, J W
Poncz, M
Sutton, M
Delgrosso, K
Schwartz, E
Surrey, S
Dworniczak, B
Mirault, M E
Paulson, K E
Matera, A G
Deka, N
Schmid, C W
Berends, T
Gamble, P E
Mullet, J E
Shimamoto, N
Ikushima, N
Utiyama, H
Tachibana, H
Horie, K
Leavitt, M C
Ito, J
Zhou, K
Brisco, P R
Hinkkanen, A E
Kohlhaw, G B
Li, P
Medon, P P
Skingle, D C
Lanser, J A
Symons, R H
Hoffmann, P U
McLaughlin, L W
Zuckermann, R
Corey, D
Schultz, P
Levinson, G
Gutman, G A
Klig, L S
Crawford, I P
Yanofsky, C
Ghosh, S S
Musso, G F
Gingeras, T R
Kwoh, D Y
Davis, G R
Koller, H T
Frondorf, K A
Maschner, P D
Vaughn, J C
Chapman, K A
Burgess, R R
Poljak, L G
Gralla, J D
Saez, L J
Gianola, K M
McNally, E M
Feghali, R
Eddy, R
Shows, T B
Leinwand, L A
Andrews, G K
Adamson, E D
Stange, N
Gross, H J
Beier, H
Wilhelm, M L
Wilhelm, F X
Hall, R M
Ksenzenko, V N
Shlyapnikov, M G
Azbarov, V G
Garcia, O
Kryukov, V M
Bayev, A A
Potter, S S
Lloyd, J A
Seto, N O
Hayashi, S
Tener, G M
author_sort Iguchi-Ariga, S M
title c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
title_short c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
title_full c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
title_fullStr c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
title_full_unstemmed c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.
title_sort c-myc protein can be substituted for sv40 t antigen in sv40 dna replication.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC305925/
https://ncbi.nlm.nih.gov/pubmed/3037484
https://ncbi.nlm.nih.gov/pmc/articles/PMC305926/
https://ncbi.nlm.nih.gov/pubmed/3037485
https://ncbi.nlm.nih.gov/pmc/articles/PMC305927/
https://ncbi.nlm.nih.gov/pubmed/3601658
https://ncbi.nlm.nih.gov/pmc/articles/PMC305928/
https://ncbi.nlm.nih.gov/pubmed/3601659
https://ncbi.nlm.nih.gov/pmc/articles/PMC305929/
https://ncbi.nlm.nih.gov/pubmed/3601660
https://ncbi.nlm.nih.gov/pmc/articles/PMC305930/
https://ncbi.nlm.nih.gov/pubmed/3299260
https://ncbi.nlm.nih.gov/pmc/articles/PMC305931/
https://ncbi.nlm.nih.gov/pubmed/3299261
https://ncbi.nlm.nih.gov/pmc/articles/PMC305932/
https://ncbi.nlm.nih.gov/pubmed/3648680
https://ncbi.nlm.nih.gov/pmc/articles/PMC305933/
https://ncbi.nlm.nih.gov/pubmed/3110742
https://ncbi.nlm.nih.gov/pmc/articles/PMC305934/
https://ncbi.nlm.nih.gov/pubmed/3601661
https://ncbi.nlm.nih.gov/pmc/articles/PMC305935/
https://ncbi.nlm.nih.gov/pubmed/3601662
https://ncbi.nlm.nih.gov/pmc/articles/PMC305936/
https://ncbi.nlm.nih.gov/pubmed/2955286
https://ncbi.nlm.nih.gov/pmc/articles/PMC305937/
https://ncbi.nlm.nih.gov/pubmed/3601663
https://ncbi.nlm.nih.gov/pmc/articles/PMC305938/
https://ncbi.nlm.nih.gov/pubmed/3299262
https://ncbi.nlm.nih.gov/pmc/articles/PMC305943/
https://ncbi.nlm.nih.gov/pubmed/3601664
https://ncbi.nlm.nih.gov/pmc/articles/PMC305944/
https://ncbi.nlm.nih.gov/pubmed/3299263
https://ncbi.nlm.nih.gov/pmc/articles/PMC305945/
https://ncbi.nlm.nih.gov/pubmed/3601665
https://ncbi.nlm.nih.gov/pmc/articles/PMC305946/
https://ncbi.nlm.nih.gov/pubmed/3037486
https://ncbi.nlm.nih.gov/pmc/articles/PMC305947/
https://ncbi.nlm.nih.gov/pubmed/3601666
https://ncbi.nlm.nih.gov/pmc/articles/PMC305948/
https://ncbi.nlm.nih.gov/pubmed/3601667
https://ncbi.nlm.nih.gov/pmc/articles/PMC305949/
https://ncbi.nlm.nih.gov/pubmed/3601668
https://ncbi.nlm.nih.gov/pmc/articles/PMC305950/
https://ncbi.nlm.nih.gov/pubmed/3037487
https://ncbi.nlm.nih.gov/pmc/articles/PMC305951/
https://ncbi.nlm.nih.gov/pubmed/3299264
https://ncbi.nlm.nih.gov/pmc/articles/PMC305952/
https://ncbi.nlm.nih.gov/pubmed/3601669
https://ncbi.nlm.nih.gov/pmc/articles/PMC305953/
https://ncbi.nlm.nih.gov/pubmed/3037488
https://ncbi.nlm.nih.gov/pmc/articles/PMC305954/
https://ncbi.nlm.nih.gov/pubmed/3601670
https://ncbi.nlm.nih.gov/pmc/articles/PMC305955/
https://ncbi.nlm.nih.gov/pubmed/3037489
https://ncbi.nlm.nih.gov/pmc/articles/PMC305956/
https://ncbi.nlm.nih.gov/pubmed/3037490
https://ncbi.nlm.nih.gov/pmc/articles/PMC305957/
https://ncbi.nlm.nih.gov/pubmed/3601671
https://ncbi.nlm.nih.gov/pmc/articles/PMC305958/
https://ncbi.nlm.nih.gov/pubmed/3299265
https://ncbi.nlm.nih.gov/pmc/articles/PMC305959/
https://ncbi.nlm.nih.gov/pubmed/3601672
https://ncbi.nlm.nih.gov/pmc/articles/PMC305960/
https://ncbi.nlm.nih.gov/pubmed/3299266
https://ncbi.nlm.nih.gov/pmc/articles/PMC305961/
https://ncbi.nlm.nih.gov/pubmed/3299267
https://ncbi.nlm.nih.gov/pmc/articles/PMC305962/
https://ncbi.nlm.nih.gov/pubmed/3299268
https://ncbi.nlm.nih.gov/pmc/articles/PMC305963/
https://ncbi.nlm.nih.gov/pubmed/3601673
https://ncbi.nlm.nih.gov/pmc/articles/PMC305964/
https://ncbi.nlm.nih.gov/pubmed/3299269
https://ncbi.nlm.nih.gov/pmc/articles/PMC305965/
https://ncbi.nlm.nih.gov/pubmed/3299270
https://ncbi.nlm.nih.gov/pmc/articles/PMC305966/
https://ncbi.nlm.nih.gov/pubmed/3601674
https://ncbi.nlm.nih.gov/pmc/articles/PMC305967/
https://ncbi.nlm.nih.gov/pubmed/3601675
https://ncbi.nlm.nih.gov/pmc/articles/PMC305968/
https://ncbi.nlm.nih.gov/pubmed/3037491
https://ncbi.nlm.nih.gov/pmc/articles/PMC305969/
https://ncbi.nlm.nih.gov/pubmed/3299271
https://ncbi.nlm.nih.gov/pmc/articles/PMC305970/
https://ncbi.nlm.nih.gov/pubmed/3037492
https://ncbi.nlm.nih.gov/pmc/articles/PMC305971/
https://ncbi.nlm.nih.gov/pubmed/3037493
https://ncbi.nlm.nih.gov/pmc/articles/PMC305972/
https://ncbi.nlm.nih.gov/pubmed/3601676
https://ncbi.nlm.nih.gov/pmc/articles/PMC305973/
https://ncbi.nlm.nih.gov/pubmed/3648681
https://ncbi.nlm.nih.gov/pmc/articles/PMC305974/
https://ncbi.nlm.nih.gov/pubmed/3601677
https://ncbi.nlm.nih.gov/pmc/articles/PMC305975/
https://ncbi.nlm.nih.gov/pubmed/3037494
https://ncbi.nlm.nih.gov/pmc/articles/PMC305976/
https://ncbi.nlm.nih.gov/pubmed/3299272
https://ncbi.nlm.nih.gov/pmc/articles/PMC305977/
https://ncbi.nlm.nih.gov/pubmed/3037495
https://ncbi.nlm.nih.gov/pmc/articles/PMC305978/
https://ncbi.nlm.nih.gov/pubmed/3110743
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