Molecular characterization of the virD operon from Agrobacterium tumefaciens.

The Agrobacterium tumefaciens Ti plasmid virulence (vir) region contains at least six transcriptional units required for the efficient transfer of T-DNA to the plant genome (virA, B, C, D, E, and G). We have reported that two proteins encoded by the 5'portion of the virD operon are required for...

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Autors principals: Porter, S G, Yanofsky, M F, Nester, E W
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Publicat: 1987
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC306264/
https://ncbi.nlm.nih.gov/pubmed/3658701
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spelling pubmed-3062642004-02-25 Molecular characterization of the virD operon from Agrobacterium tumefaciens. Porter, S G Yanofsky, M F Nester, E W Nucleic Acids Res The Agrobacterium tumefaciens Ti plasmid virulence (vir) region contains at least six transcriptional units required for the efficient transfer of T-DNA to the plant genome (virA, B, C, D, E, and G). We have reported that two proteins encoded by the 5'portion of the virD operon are required for a site-specific endonuclease activity that nicks the direct repeats which flank the T-DNA. We have presented the nucleotide sequence for this portion of the operon. The nucleotide sequence of the remainder of the virD operon essential for virulence has now been determined. Two additional open reading frames encode proteins of 21.3 and 75.8 kilodaltons (kd). Translational fusions between virD2, virD3, and virD4 proteins and trpE produced fusion proteins of the size predicted from the nucleotide sequence data. We have used antisera directed against the trpE-virD2 fusion protein to detect both native virD2 protein and a virD2-lacZ fusion protein in crude extracts from Agrobacterium. 1987-09-25 /pmc/articles/PMC306264/ /pubmed/3658701 Text en
institution US National Library of Medicine
collection PubMed Central
language en
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description The Agrobacterium tumefaciens Ti plasmid virulence (vir) region contains at least six transcriptional units required for the efficient transfer of T-DNA to the plant genome (virA, B, C, D, E, and G). We have reported that two proteins encoded by the 5'portion of the virD operon are required for a site-specific endonuclease activity that nicks the direct repeats which flank the T-DNA. We have presented the nucleotide sequence for this portion of the operon. The nucleotide sequence of the remainder of the virD operon essential for virulence has now been determined. Two additional open reading frames encode proteins of 21.3 and 75.8 kilodaltons (kd). Translational fusions between virD2, virD3, and virD4 proteins and trpE produced fusion proteins of the size predicted from the nucleotide sequence data. We have used antisera directed against the trpE-virD2 fusion protein to detect both native virD2 protein and a virD2-lacZ fusion protein in crude extracts from Agrobacterium.
author Porter, S G
Yanofsky, M F
Nester, E W
spellingShingle Porter, S G
Yanofsky, M F
Nester, E W
Molecular characterization of the virD operon from Agrobacterium tumefaciens.
author_facet Porter, S G
Yanofsky, M F
Nester, E W
author_sort Porter, S G
title Molecular characterization of the virD operon from Agrobacterium tumefaciens.
title_short Molecular characterization of the virD operon from Agrobacterium tumefaciens.
title_full Molecular characterization of the virD operon from Agrobacterium tumefaciens.
title_fullStr Molecular characterization of the virD operon from Agrobacterium tumefaciens.
title_full_unstemmed Molecular characterization of the virD operon from Agrobacterium tumefaciens.
title_sort molecular characterization of the vird operon from agrobacterium tumefaciens.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC306264/
https://ncbi.nlm.nih.gov/pubmed/3658701
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