Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana

The seeds of higher plants accumulate large quantities of storage protein. During seed maturation, storage protein precursors synthesized on rough endoplasmic reticulum are sorted to protein storage vacuoles, where they are converted into the mature forms and accumulated. Previous attempts to determ...

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主要な著者: Shimada, Tomoo, Fuji, Kentaro, Tamura, Kentaro, Kondo, Maki, Nishimura, Mikio, Hara-Nishimura, Ikuko
フォーマット: 論文
言語:en
出版事項: National Academy of Sciences 2003
主題:
Biological Sciences
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC307698/
https://ncbi.nlm.nih.gov/pubmed/14657332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2530568100
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spelling pubmed-3076982004-06-23 Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana Shimada, Tomoo Fuji, Kentaro Tamura, Kentaro Kondo, Maki Nishimura, Mikio Hara-Nishimura, Ikuko Proc Natl Acad Sci U S A Biological Sciences The seeds of higher plants accumulate large quantities of storage protein. During seed maturation, storage protein precursors synthesized on rough endoplasmic reticulum are sorted to protein storage vacuoles, where they are converted into the mature forms and accumulated. Previous attempts to determine the sorting machinery for storage proteins have not been successful. Here we show that a type I membrane protein, AtVSR1/AtELP, of Arabidopsis functions as a sorting receptor for storage proteins. The atvsr1 mutant missorts storage proteins by secreting them from cells, resulting in an enlarged and electron-dense extracellular space in the seeds. The atvsr1 seeds have distorted cells and smaller protein storage vacuoles than do WT seeds, and atvsr1 seeds abnormally accumulate the precursors of two major storage proteins, 12S globulin and 2S albumin, together with the mature forms of these proteins. AtVSR1 was found to bind to the C-terminal peptide of 12S globulin in a Ca(2+)-dependent manner. These findings demonstrate a receptor-mediated transport of seed storage proteins to protein storage vacuoles in higher plants. National Academy of Sciences 2003-12-23 2003-12-04 /pmc/articles/PMC307698/ /pubmed/14657332 http://dx.doi.org/10.1073/pnas.2530568100 Text en Copyright © 2003, The National Academy of Sciences
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Biological Sciences
spellingShingle Biological Sciences
Shimada, Tomoo
Fuji, Kentaro
Tamura, Kentaro
Kondo, Maki
Nishimura, Mikio
Hara-Nishimura, Ikuko
Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
description The seeds of higher plants accumulate large quantities of storage protein. During seed maturation, storage protein precursors synthesized on rough endoplasmic reticulum are sorted to protein storage vacuoles, where they are converted into the mature forms and accumulated. Previous attempts to determine the sorting machinery for storage proteins have not been successful. Here we show that a type I membrane protein, AtVSR1/AtELP, of Arabidopsis functions as a sorting receptor for storage proteins. The atvsr1 mutant missorts storage proteins by secreting them from cells, resulting in an enlarged and electron-dense extracellular space in the seeds. The atvsr1 seeds have distorted cells and smaller protein storage vacuoles than do WT seeds, and atvsr1 seeds abnormally accumulate the precursors of two major storage proteins, 12S globulin and 2S albumin, together with the mature forms of these proteins. AtVSR1 was found to bind to the C-terminal peptide of 12S globulin in a Ca(2+)-dependent manner. These findings demonstrate a receptor-mediated transport of seed storage proteins to protein storage vacuoles in higher plants.
author Shimada, Tomoo
Fuji, Kentaro
Tamura, Kentaro
Kondo, Maki
Nishimura, Mikio
Hara-Nishimura, Ikuko
author_facet Shimada, Tomoo
Fuji, Kentaro
Tamura, Kentaro
Kondo, Maki
Nishimura, Mikio
Hara-Nishimura, Ikuko
author_sort Shimada, Tomoo
title Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
title_short Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
title_full Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
title_fullStr Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
title_full_unstemmed Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana
title_sort vacuolar sorting receptor for seed storage proteins in arabidopsis thaliana
publisher National Academy of Sciences
publisher_facet National Academy of Sciences
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC307698/
https://ncbi.nlm.nih.gov/pubmed/14657332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2530568100
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