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Tritium labeling of antisense oligonucleotides by exchange with tritiated water.
We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the sta...
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| 主要な著者: | , , , , , |
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| フォーマット: | 論文 |
| 言語: | en |
| 出版事項: |
1993
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309879/ https://ncbi.nlm.nih.gov/pubmed/8367289 |
| タグ: |
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