Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

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Hlavní autoři: Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Médium: Článek
Jazyk:en
Vydáno: Oxford University Press 2000
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Articles
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC310236/
https://ncbi.nlm.nih.gov/pubmed/10747035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.7.1681
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https://ncbi.nlm.nih.gov/pmc/articles/PMC310236/
https://ncbi.nlm.nih.gov/pubmed/10747035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.7.1681

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