Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.

Murine erythroleukemia cells (MELC) can be induced to express the characteristics of erythroid differentiation by a variety of agents. Previous studies indicate that an action of inducer, occurring during early S phase, may be critical to the expression of differentiated characteristics such as init...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Epner, E, Rifkind, R A, Marks, P A
Formato: Artigo
Idioma:en
Publicado em: 1981
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC319499/
https://ncbi.nlm.nih.gov/pubmed/6942415
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-319499
record_format dspace
spelling pubmed-3194992004-03-13 Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells. Epner, E Rifkind, R A Marks, P A Proc Natl Acad Sci U S A Research Article Murine erythroleukemia cells (MELC) can be induced to express the characteristics of erythroid differentiation by a variety of agents. Previous studies indicate that an action of inducer, occurring during early S phase, may be critical to the expression of differentiated characteristics such as initiation of accumulation of newly synthesized alpha and beta globin mRNAs. In this investigation, the time of replication of globin genes in MELC was studied. DNA was isolated from synchronous populations of cells obtained by centrifugal elutriation. Newly replicated DNA sequences were prepared from synchronized cells cultured for 1 1/2 hr with 5-bromodeoxyuridine; bromodeoxyuridine-containing DNA was isolated by CsCl gradient centrifugation. By employing cloned probes for hybridization to newly synthesized DNA, it was found that alpha and beta globin gene sequences are replicated early in S phase, while ribosomal RNA gene sequences are replicated to about the same extent in early, middle, and late S phases. 1981-05 /pmc/articles/PMC319499/ /pubmed/6942415 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Epner, E
Rifkind, R A
Marks, P A
Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
description Murine erythroleukemia cells (MELC) can be induced to express the characteristics of erythroid differentiation by a variety of agents. Previous studies indicate that an action of inducer, occurring during early S phase, may be critical to the expression of differentiated characteristics such as initiation of accumulation of newly synthesized alpha and beta globin mRNAs. In this investigation, the time of replication of globin genes in MELC was studied. DNA was isolated from synchronous populations of cells obtained by centrifugal elutriation. Newly replicated DNA sequences were prepared from synchronized cells cultured for 1 1/2 hr with 5-bromodeoxyuridine; bromodeoxyuridine-containing DNA was isolated by CsCl gradient centrifugation. By employing cloned probes for hybridization to newly synthesized DNA, it was found that alpha and beta globin gene sequences are replicated early in S phase, while ribosomal RNA gene sequences are replicated to about the same extent in early, middle, and late S phases.
author Epner, E
Rifkind, R A
Marks, P A
author_facet Epner, E
Rifkind, R A
Marks, P A
author_sort Epner, E
title Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
title_short Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
title_full Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
title_fullStr Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
title_full_unstemmed Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
title_sort replication of alpha and beta globin dna sequences occurs during early s phase in murine erythroleukemia cells.
publishDate 1981
url https://ncbi.nlm.nih.gov/pmc/articles/PMC319499/
https://ncbi.nlm.nih.gov/pubmed/6942415
_version_ 1759377928049655808

Registos relacionados