The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.

The sequence of maize chloroplast tRNAIle2, encoded in the 16S to 23S rDNA spacer, was determined using in vitro labeling techniques. The sequence is: pG-G-G-C-U-A-U-U-A-G-C-U-C-A-G-U-Gm-G-D-A-G-A-G-C-m22G-C-G-C-C-C-C-U-G-A-U-t6A- A-G-G-G-C-G-A-G-m7G-acp3U-C-U-C-U-G-G-T-psi-C-A-A-G-U-C-C-A-G-G-A-U-G...

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Main Authors: Guillemaut, P, Weil, J H
Formato: Artigo
Idioma:en
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320556/
https://ncbi.nlm.nih.gov/pubmed/6917975
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spelling pubmed-3205562004-02-10 The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer. Guillemaut, P Weil, J H Nucleic Acids Res Research Article The sequence of maize chloroplast tRNAIle2, encoded in the 16S to 23S rDNA spacer, was determined using in vitro labeling techniques. The sequence is: pG-G-G-C-U-A-U-U-A-G-C-U-C-A-G-U-Gm-G-D-A-G-A-G-C-m22G-C-G-C-C-C-C-U-G-A-U-t6A- A-G-G-G-C-G-A-G-m7G-acp3U-C-U-C-U-G-G-T-psi-C-A-A-G-U-C-C-A-G-G-A-U-G-G-C-C-C-A -C-C-AOH. This sequence is identical to that predicted from the corresponding gene sequence, after excision of a long intervening sequence (1), but shows the post-transcriptional modifications of this tRNA. Furthermore it demonstrates that the excision of the intron occurs after the second base following the anticodon and that this gene, which is over 1000 base-pair long, is transcribed and processed into a mature functional chloroplast-tRNA. The sequence of maize (a monocot) and spinach (a dicot) tRNAIle2 are shown to be identical. 1982-03-11 /pmc/articles/PMC320556/ /pubmed/6917975 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Guillemaut, P
Weil, J H
The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
description The sequence of maize chloroplast tRNAIle2, encoded in the 16S to 23S rDNA spacer, was determined using in vitro labeling techniques. The sequence is: pG-G-G-C-U-A-U-U-A-G-C-U-C-A-G-U-Gm-G-D-A-G-A-G-C-m22G-C-G-C-C-C-C-U-G-A-U-t6A- A-G-G-G-C-G-A-G-m7G-acp3U-C-U-C-U-G-G-T-psi-C-A-A-G-U-C-C-A-G-G-A-U-G-G-C-C-C-A -C-C-AOH. This sequence is identical to that predicted from the corresponding gene sequence, after excision of a long intervening sequence (1), but shows the post-transcriptional modifications of this tRNA. Furthermore it demonstrates that the excision of the intron occurs after the second base following the anticodon and that this gene, which is over 1000 base-pair long, is transcribed and processed into a mature functional chloroplast-tRNA. The sequence of maize (a monocot) and spinach (a dicot) tRNAIle2 are shown to be identical.
author Guillemaut, P
Weil, J H
author_facet Guillemaut, P
Weil, J H
author_sort Guillemaut, P
title The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
title_short The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
title_full The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
title_fullStr The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
title_full_unstemmed The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.
title_sort nucleotide sequence of the maize and spinach chloroplast isoleucine transfer rna encoded in the 16s to 23s rdna spacer.
publishDate 1982
url https://ncbi.nlm.nih.gov/pmc/articles/PMC320556/
https://ncbi.nlm.nih.gov/pubmed/6917975
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