Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.

Two cDNA libraries were constructed, using respectively the 12S and the 16S sucrose gradient fractions of polysomal poly (A)+ RNA from mouse C243 cells induced with Newcastle disease virus. Screening of a part of both libraries by mRNA selection hybridization assays revealed the presence of two plas...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Skup, D, Windass, J D, Sor, F, George, H, Williams, B R, Fukuhara, H, De Maeyer-Guignard, J, De Maeyer, E
Formato: Artigo
Idioma:en
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320691/
https://ncbi.nlm.nih.gov/pubmed/6179042
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-320691
record_format dspace
spelling pubmed-3206912004-02-10 Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs. Skup, D Windass, J D Sor, F George, H Williams, B R Fukuhara, H De Maeyer-Guignard, J De Maeyer, E Nucleic Acids Res Research Article Two cDNA libraries were constructed, using respectively the 12S and the 16S sucrose gradient fractions of polysomal poly (A)+ RNA from mouse C243 cells induced with Newcastle disease virus. Screening of a part of both libraries by mRNA selection hybridization assays revealed the presence of two plasmids hybridizing to an mRNA, whose translation product was characterized as mouse IFN-beta. Blot analysis of RNA indicated that mRNA hybridizing to the DNA from both plasmids could be detected in induced but not in uninduced C243 cells. The two cDNA inserts did not cross hybridize and had distinct restriction maps. Sequencing revealed that both inserts represented the end of the coding region and the entire 3' non coding region of two district mRNAs. Although different, the putative 39 AA and 65 AA carboxy termini of both Mu IFN-beta s display some homology to human IFN-beta 1. Thus there are at least two different murine IFN-beta genes. 1982-05-25 /pmc/articles/PMC320691/ /pubmed/6179042 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Skup, D
Windass, J D
Sor, F
George, H
Williams, B R
Fukuhara, H
De Maeyer-Guignard, J
De Maeyer, E
Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
description Two cDNA libraries were constructed, using respectively the 12S and the 16S sucrose gradient fractions of polysomal poly (A)+ RNA from mouse C243 cells induced with Newcastle disease virus. Screening of a part of both libraries by mRNA selection hybridization assays revealed the presence of two plasmids hybridizing to an mRNA, whose translation product was characterized as mouse IFN-beta. Blot analysis of RNA indicated that mRNA hybridizing to the DNA from both plasmids could be detected in induced but not in uninduced C243 cells. The two cDNA inserts did not cross hybridize and had distinct restriction maps. Sequencing revealed that both inserts represented the end of the coding region and the entire 3' non coding region of two district mRNAs. Although different, the putative 39 AA and 65 AA carboxy termini of both Mu IFN-beta s display some homology to human IFN-beta 1. Thus there are at least two different murine IFN-beta genes.
author Skup, D
Windass, J D
Sor, F
George, H
Williams, B R
Fukuhara, H
De Maeyer-Guignard, J
De Maeyer, E
author_facet Skup, D
Windass, J D
Sor, F
George, H
Williams, B R
Fukuhara, H
De Maeyer-Guignard, J
De Maeyer, E
author_sort Skup, D
title Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
title_short Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
title_full Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
title_fullStr Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
title_full_unstemmed Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs.
title_sort molecular cloning of partial cdna copies of two distinct mouse ifn-beta mrnas.
publishDate 1982
url https://ncbi.nlm.nih.gov/pmc/articles/PMC320691/
https://ncbi.nlm.nih.gov/pubmed/6179042
_version_ 1759378098488344576

Registos relacionados