Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.

We have studied the effect of intermolecular triplexes formation on the yield of cyclobutane photodimers in DNA. DNA duplex within the pyrimidine-purine-pyrimidine triplex d(TC)nd(GA)nd(CT)n is protected from the formation of cyclobutane photodimers in the case of the stabilization of this triplex b...

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Príomhchruthaitheoirí: Malkov, V A, Soyfer, V N, Frank-Kamenetskii, M D
Formáid: Alt
Teanga:en
Foilsithe / Cruthaithe: 1992
Rochtain ar líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC334247/
https://ncbi.nlm.nih.gov/pubmed/1408804
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spelling pubmed-3342472004-02-10 Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA. Malkov, V A Soyfer, V N Frank-Kamenetskii, M D Nucleic Acids Res We have studied the effect of intermolecular triplexes formation on the yield of cyclobutane photodimers in DNA. DNA duplex within the pyrimidine-purine-pyrimidine triplex d(TC)nd(GA)nd(CT)n is protected from the formation of cyclobutane photodimers in the case of the stabilization of this triplex by acid pH, and in the case of supplementary stabilization by Mg2+ or Zn2+. We have studied pH-independent pyrimidine-purine-purine triplexes stabilized by bivalent cations. In such triplexes, the protection from the formation of [6-4] photodimers is observed, whereas the protection from cyclobutane dimer formation does not take place. The formation of the d(TC)nd(GA)nd(GA)n triplex leads to an inversion of the intensities of cyclobutane CT and TC photodimers. We observed a sharp decrease in photoreactivity with respect to cyclobutane dimers in the duplex tract d(C)18d(G)18 in the presence of Ba2+, Cd2+, Co2+, Mn2+, Zn2+ and Ni2+. The formation of the d(C)nd(G)nd(G)n triplex leads to 'antifootprinting', i.e. an increase in the yield of cyclobutane photodimers. 1992-09-25 /pmc/articles/PMC334247/ /pubmed/1408804 Text en
institution US National Library of Medicine
collection PubMed Central
language en
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description We have studied the effect of intermolecular triplexes formation on the yield of cyclobutane photodimers in DNA. DNA duplex within the pyrimidine-purine-pyrimidine triplex d(TC)nd(GA)nd(CT)n is protected from the formation of cyclobutane photodimers in the case of the stabilization of this triplex by acid pH, and in the case of supplementary stabilization by Mg2+ or Zn2+. We have studied pH-independent pyrimidine-purine-purine triplexes stabilized by bivalent cations. In such triplexes, the protection from the formation of [6-4] photodimers is observed, whereas the protection from cyclobutane dimer formation does not take place. The formation of the d(TC)nd(GA)nd(GA)n triplex leads to an inversion of the intensities of cyclobutane CT and TC photodimers. We observed a sharp decrease in photoreactivity with respect to cyclobutane dimers in the duplex tract d(C)18d(G)18 in the presence of Ba2+, Cd2+, Co2+, Mn2+, Zn2+ and Ni2+. The formation of the d(C)nd(G)nd(G)n triplex leads to 'antifootprinting', i.e. an increase in the yield of cyclobutane photodimers.
author Malkov, V A
Soyfer, V N
Frank-Kamenetskii, M D
spellingShingle Malkov, V A
Soyfer, V N
Frank-Kamenetskii, M D
Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
author_facet Malkov, V A
Soyfer, V N
Frank-Kamenetskii, M D
author_sort Malkov, V A
title Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
title_short Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
title_full Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
title_fullStr Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
title_full_unstemmed Effect of intermolecular triplex formation on the yield of cyclobutane photodimers in DNA.
title_sort effect of intermolecular triplex formation on the yield of cyclobutane photodimers in dna.
publishDate 1992
url https://ncbi.nlm.nih.gov/pmc/articles/PMC334247/
https://ncbi.nlm.nih.gov/pubmed/1408804
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