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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such bioti...
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| 主要な著者: | , , , |
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| フォーマット: | 論文 |
| 言語: | en |
| 出版事項: |
1985
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340973/ https://ncbi.nlm.nih.gov/pubmed/2582352 |
| タグ: |
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| 要約: | Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied. |
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