Variation in the polyadenylylation site of bovine prolactin mRNA.

The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragm...

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Main Authors: Sasavage, N L, Smith, M, Gillam, S, Woychik, R P, Rottman, F M
Formato: Artigo
Idioma:en
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC345698/
https://ncbi.nlm.nih.gov/pubmed/6952180
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spelling pubmed-3456982004-03-13 Variation in the polyadenylylation site of bovine prolactin mRNA. Sasavage, N L Smith, M Gillam, S Woychik, R P Rottman, F M Proc Natl Acad Sci U S A Research Article The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three d(pT8-N-N') sequences. Comparison of the sequence bands produced by initiation of cDNA synthesis on the bPRL mRNA template with d(pT8-A-G), d(pT8-G-A), and d(pT8-C-G) revealed a shifted pattern of identical fragments. The shift in position of related sequence bands on the gel suggested that the difference in length of the three major bPRL mRNA species occurred within a span of 12 nucleotides. Sequence analysis conducted with the three d(pT8-N-N') primers gave identical nucleotide sequences for the 3' noncoding region of the bPRL mRNA species and suggested that the mRNA molecules were heterogeneous in length. The existence of multiple poly(A) sites was confirmed by determination of the nucleotide sequence of bPRL cDNA clones containing two of the major poly(A)-adjacent sequences predicted by the oligodeoxynucleotide primers. The mRNA molecules containing these multiple poly(A)-addition sites were shown to be present in the bPRL mRNA obtained from a single pituitary gland. The variation in the poly(A) junction of bPRL mRNA may be a reflection of the processing events at the 3' terminus of mRNAs. 1982-01 /pmc/articles/PMC345698/ /pubmed/6952180 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Sasavage, N L
Smith, M
Gillam, S
Woychik, R P
Rottman, F M
Variation in the polyadenylylation site of bovine prolactin mRNA.
description The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three d(pT8-N-N') sequences. Comparison of the sequence bands produced by initiation of cDNA synthesis on the bPRL mRNA template with d(pT8-A-G), d(pT8-G-A), and d(pT8-C-G) revealed a shifted pattern of identical fragments. The shift in position of related sequence bands on the gel suggested that the difference in length of the three major bPRL mRNA species occurred within a span of 12 nucleotides. Sequence analysis conducted with the three d(pT8-N-N') primers gave identical nucleotide sequences for the 3' noncoding region of the bPRL mRNA species and suggested that the mRNA molecules were heterogeneous in length. The existence of multiple poly(A) sites was confirmed by determination of the nucleotide sequence of bPRL cDNA clones containing two of the major poly(A)-adjacent sequences predicted by the oligodeoxynucleotide primers. The mRNA molecules containing these multiple poly(A)-addition sites were shown to be present in the bPRL mRNA obtained from a single pituitary gland. The variation in the poly(A) junction of bPRL mRNA may be a reflection of the processing events at the 3' terminus of mRNAs.
author Sasavage, N L
Smith, M
Gillam, S
Woychik, R P
Rottman, F M
author_facet Sasavage, N L
Smith, M
Gillam, S
Woychik, R P
Rottman, F M
author_sort Sasavage, N L
title Variation in the polyadenylylation site of bovine prolactin mRNA.
title_short Variation in the polyadenylylation site of bovine prolactin mRNA.
title_full Variation in the polyadenylylation site of bovine prolactin mRNA.
title_fullStr Variation in the polyadenylylation site of bovine prolactin mRNA.
title_full_unstemmed Variation in the polyadenylylation site of bovine prolactin mRNA.
title_sort variation in the polyadenylylation site of bovine prolactin mrna.
publishDate 1982
url https://ncbi.nlm.nih.gov/pmc/articles/PMC345698/
https://ncbi.nlm.nih.gov/pubmed/6952180
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