Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.

Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 8...

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Main Authors: Wu, W C, Walaas, S I, Nairn, A C, Greengard, P
Formato: Artigo
Idioma:en
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC346873/
https://ncbi.nlm.nih.gov/pubmed/6957862
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spelling pubmed-3468732004-03-13 Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes. Wu, W C Walaas, S I Nairn, A C Greengard, P Proc Natl Acad Sci U S A Research Article Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 87,000 substrate protein is stimulated by depolarization-induced calcium influx. Phosphorylation of this Mr 87,000 substrate occurred in synaptosomal cytosol and was markedly stimulated by calcium/phosphatidylserine. Calmodulin inhibited this phosphorylation reaction. This substrate for calcium/phospholipid-dependent protein kinase is enriched in and appears to be specific to neurons. 1982-09 /pmc/articles/PMC346873/ /pubmed/6957862 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Wu, W C
Walaas, S I
Nairn, A C
Greengard, P
Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
description Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 87,000 substrate protein is stimulated by depolarization-induced calcium influx. Phosphorylation of this Mr 87,000 substrate occurred in synaptosomal cytosol and was markedly stimulated by calcium/phosphatidylserine. Calmodulin inhibited this phosphorylation reaction. This substrate for calcium/phospholipid-dependent protein kinase is enriched in and appears to be specific to neurons.
author Wu, W C
Walaas, S I
Nairn, A C
Greengard, P
author_facet Wu, W C
Walaas, S I
Nairn, A C
Greengard, P
author_sort Wu, W C
title Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
title_short Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
title_full Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
title_fullStr Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
title_full_unstemmed Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.
title_sort calcium/phospholipid regulates phosphorylation of a mr "87k" substrate protein in brain synaptosomes.
publishDate 1982
url https://ncbi.nlm.nih.gov/pmc/articles/PMC346873/
https://ncbi.nlm.nih.gov/pubmed/6957862
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