Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity

Three mutants of the crucifer Arabidopsis thaliana (Linnaeus) Heynhold were isolated that are completely lacking in activity catalyzed by serine-glyoxylate aminotransferase (EC 2.6.1.45), a peroxisomal enzyme involved in photorespiratory carbon metabolism. These mutants were viable and exhibited nor...

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Main Authors: Somerville, C. R., Ogren, W. L.
Formato: Artigo
Idioma:en
Publicado em: 1980
Assuntos:
Biological Sciences: Botany
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC349467/
https://ncbi.nlm.nih.gov/pubmed/16592821
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spelling pubmed-3494672004-03-13 Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity Somerville, C. R. Ogren, W. L. Proc Natl Acad Sci U S A Biological Sciences: Botany Three mutants of the crucifer Arabidopsis thaliana (Linnaeus) Heynhold were isolated that are completely lacking in activity catalyzed by serine-glyoxylate aminotransferase (EC 2.6.1.45), a peroxisomal enzyme involved in photorespiratory carbon metabolism. These mutants were viable and exhibited normal photosynthesis under conditions that suppressed photorespiration, but they were inviable and photosynthesized at greatly reduced rates under conditions that promoted photorespiration. Serine and glycine accumulated as end products of photosynthesis in the mutants, mostly at the expense of starch and sucrose. The mutants are allelic, and the segregation patterns of plant viability, photosynthetic activity, and enzyme activity in the F(1) and F(2) generations indicated that all the observed effects were caused by a single recessive nuclear mutation. This conclusion was confirmed by the isolation of seven revertants in which viability, photosynthetic capacity, and enzyme activity were simultaneously restored. Mutants of the type described here, in which photorespiration is changed from a merely wasteful process into one that is lethal, may permit the direct selection of secondary mutations that reduce photorespiration. 1980-05 /pmc/articles/PMC349467/ /pubmed/16592821 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Biological Sciences: Botany
spellingShingle Biological Sciences: Botany
Somerville, C. R.
Ogren, W. L.
Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
description Three mutants of the crucifer Arabidopsis thaliana (Linnaeus) Heynhold were isolated that are completely lacking in activity catalyzed by serine-glyoxylate aminotransferase (EC 2.6.1.45), a peroxisomal enzyme involved in photorespiratory carbon metabolism. These mutants were viable and exhibited normal photosynthesis under conditions that suppressed photorespiration, but they were inviable and photosynthesized at greatly reduced rates under conditions that promoted photorespiration. Serine and glycine accumulated as end products of photosynthesis in the mutants, mostly at the expense of starch and sucrose. The mutants are allelic, and the segregation patterns of plant viability, photosynthetic activity, and enzyme activity in the F(1) and F(2) generations indicated that all the observed effects were caused by a single recessive nuclear mutation. This conclusion was confirmed by the isolation of seven revertants in which viability, photosynthetic capacity, and enzyme activity were simultaneously restored. Mutants of the type described here, in which photorespiration is changed from a merely wasteful process into one that is lethal, may permit the direct selection of secondary mutations that reduce photorespiration.
author Somerville, C. R.
Ogren, W. L.
author_facet Somerville, C. R.
Ogren, W. L.
author_sort Somerville, C. R.
title Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
title_short Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
title_full Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
title_fullStr Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
title_full_unstemmed Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
title_sort photorespiration mutants of arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity
publishDate 1980
url https://ncbi.nlm.nih.gov/pmc/articles/PMC349467/
https://ncbi.nlm.nih.gov/pubmed/16592821
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